Cardiac troponin I carryover by very high patient samples still causes false-positive results on the Beckman Coulter AccuTnI + 3

Wilgen, Urs, Pretorius, Carel J., Gould, Michael J. and Ungerer, Jacobus P. J. (2016) Cardiac troponin I carryover by very high patient samples still causes false-positive results on the Beckman Coulter AccuTnI + 3. Annals of Clinical Biochemistry, 53 1: 177-179. doi:10.1177/0004563215606739


Author Wilgen, Urs
Pretorius, Carel J.
Gould, Michael J.
Ungerer, Jacobus P. J.
Title Cardiac troponin I carryover by very high patient samples still causes false-positive results on the Beckman Coulter AccuTnI + 3
Journal name Annals of Clinical Biochemistry   Check publisher's open access policy
ISSN 1758-1001
0004-5632
Publication date 2016-01-01
Year available 2015
Sub-type Article (original research)
DOI 10.1177/0004563215606739
Open Access Status Not Open Access
Volume 53
Issue 1
Start page 177
End page 179
Total pages 3
Place of publication London, United Kingdom
Publisher Sage Publications
Language eng
Formatted abstract
Background: False-positive cardiac troponin I results as a result of carryover have previously been reported on the Beckman Coulter AccuTnI assay. We sought to determine if the carryover problem had been resolved with the new AccuTnI + 3 assay.
Methods: Carryover experiments were performed in parallel on the Beckman Coulter Access2 analyser using the legacy AccuTnI and new AccuTnI + 3 assays. The same negative patient pool sample was analysed before and after a single analysis of an extremely elevated patient sample.
Results: Analysis of a single extremely high sample caused elevations above the 99th percentile cut-off, and thus false-positive cardiac troponin I results on both assays. Both assays demonstrated carryover and subsequent further elevations in negative pool results the following day.
Conclusions: Our study replicates our previously published findings of carryover and reagent pack contamination on the AccuTnI assay. Despite improvements on the new AccuTnI + 3 assay, carryover and reagent pack contamination are still present.
Keyword Analytical systems
Immunoassay
Troponin
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published online 7 September 2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Medicine Publications
 
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