Non-Animal Models of Epithelial Barriers (Skin, Intestine and Lung) in Research, Industrial Applications and Regulatory Toxicology

Gordon, Sarah, Daneshian, Mardas, Bouwstra, Joke, Caloni, Francesca, Constant, Samuel, Davies, Donna E., Dandekar, Gudrun, Guzman, Carlos A., Fabian, Eric, Haltner, Eleonore, Hartung, Thomas, Hasiwa, Nina, Hayden, Patrick, Kandarova, Helena, Khare, Sangeeta, Krug, Harald F., Kneuer, Carsten, Leist, Marcel, Lian, Guoping, Marx, Uwe, Metzger, Marco, Ott, Katharina, Prieto, Pilar, Roberts, Michael S., Roggen, Erwin L., Tralau, Tewes, van den Braak, Claudia, Walles, Heike and Lehr, Claus-Michael (2015) Non-Animal Models of Epithelial Barriers (Skin, Intestine and Lung) in Research, Industrial Applications and Regulatory Toxicology. Altex-Alternatives to Animal Experimentation, 32 4: 327-378. doi:10.14573/altex.1510051

Author Gordon, Sarah
Daneshian, Mardas
Bouwstra, Joke
Caloni, Francesca
Constant, Samuel
Davies, Donna E.
Dandekar, Gudrun
Guzman, Carlos A.
Fabian, Eric
Haltner, Eleonore
Hartung, Thomas
Hasiwa, Nina
Hayden, Patrick
Kandarova, Helena
Khare, Sangeeta
Krug, Harald F.
Kneuer, Carsten
Leist, Marcel
Lian, Guoping
Marx, Uwe
Metzger, Marco
Ott, Katharina
Prieto, Pilar
Roberts, Michael S.
Roggen, Erwin L.
Tralau, Tewes
van den Braak, Claudia
Walles, Heike
Lehr, Claus-Michael
Title Non-Animal Models of Epithelial Barriers (Skin, Intestine and Lung) in Research, Industrial Applications and Regulatory Toxicology
Journal name Altex-Alternatives to Animal Experimentation   Check publisher's open access policy
ISSN 1868-596X
Publication date 2015-01-01
Sub-type Article (original research)
DOI 10.14573/altex.1510051
Open Access Status DOI
Volume 32
Issue 4
Start page 327
End page 378
Total pages 52
Place of publication Heidelberg, Germany
Publisher Springer Spektrum
Language eng
Abstract Models of the outer epithelia of the human body - namely the skin, the intestine and the lung - have found valid applications in both research and industrial settings as attractive alternatives to animal testing. A variety of approaches to model these barriers are currently employed in such fields, ranging from the utilization of ex vivo tissue to reconstructed in vitro models, and further to chip-based technologies, synthetic membrane systems and, of increasing current interest, in silico modeling approaches. An international group of experts in the field of epithelial barriers was convened from academia, industry and regulatory bodies to present both the current state of the art of non-animal models of the skin, intestinal and pulmonary barriers in their various fields of application, and to discuss research-based, industry-driven and regulatory-relevant future directions for both the development of new models and the refinement of existing test methods. Issues of model relevance and preference, validation and standardization, acceptance, and the need for simplicity versus complexity were focal themes of the discussions. The outcomes of workshop presentations and discussions, in relation to both current status and future directions in the utilization and development of epithelial barrier models, are presented by the attending experts in the current report.
Keyword in vitro models
epithelial cell culture
transport studies
In-Vitro Model
Air-Liquid Interface
Embryonic Stem-Cells
Artificial Membrane-Permeability
Reconstructed Human Epidermis
Respiratory Syncytial Virus
Pulmonary Drug-Delivery
Vesicle-Based Barrier
Asthmatic Bronchial Epithelium
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID G0900453
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
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