Measuring the interaction forces between protein inclusion bodies and an air bubble using an atomic force microscope

Wangsa-Wirawan, N. D., Ikai, A., ONeill, B. K. and Middelberg, A. P. J. (2001) Measuring the interaction forces between protein inclusion bodies and an air bubble using an atomic force microscope. Biotechnology Progress, 17 5: 963-969. doi:10.1021/bp010072+


Author Wangsa-Wirawan, N. D.
Ikai, A.
ONeill, B. K.
Middelberg, A. P. J.
Title Measuring the interaction forces between protein inclusion bodies and an air bubble using an atomic force microscope
Journal name Biotechnology Progress   Check publisher's open access policy
ISSN 8756-7938
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1021/bp010072+
Volume 17
Issue 5
Start page 963
End page 969
Total pages 7
Place of publication Washington
Publisher Amer Chemical Soc
Language eng
Abstract Interaction forces between protein inclusion bodies and an air bubble have been quantified using an atomic force microscope (AFM). The inclusion bodies were attached to the AFM tip by covalent bonds. Interaction forces measured in various buffer concentrations varied from 9.7 nN to 25.3 nN (+/- 4-11%) depending on pH. Hydrophobic forces provide a stronger contribution to overall interaction force than electrostatic double layer forces. It also appears that the ionic strength affects the interaction force in a complex way that cannot be directly predicted by DLVO theory. The effects of pH are significantly stronger for the inclusion body compared to the air bubble. This study provides fundamental information that will subsequently facilitate the rational design of flotation recovery system for inclusion bodies. It has also demonstrated the potential of AFM to facilitate the design of such processes from a practical viewpoint.
Keyword Biotechnology & Applied Microbiology
Food Science & Technology
Inorganic Salt-solutions
Surface Interactions
Aqueous-electrolyte
Colloidal Forces
Adhesion
Particle
Silica
Water
Reversal
Friction
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 13 Aug 2007, 22:35:38 EST