Identification of genes implicated in toxin production in the cyanobacterium Cylindrospermopsis raciborskii

Schembri, M. A., Neilan, B. A. and Saint, C. P. (2001) Identification of genes implicated in toxin production in the cyanobacterium Cylindrospermopsis raciborskii. Environmental Toxicology, 16 5: 413-421. doi:10.1002/tox.1051


Author Schembri, M. A.
Neilan, B. A.
Saint, C. P.
Title Identification of genes implicated in toxin production in the cyanobacterium Cylindrospermopsis raciborskii
Journal name Environmental Toxicology   Check publisher's open access policy
ISSN 1520-4081
Publication date 2001-10-01
Year available 2001
Sub-type Article (original research)
DOI 10.1002/tox.1051
Open Access Status Not yet assessed
Volume 16
Issue 5
Start page 413
End page 421
Total pages 9
Place of publication New York
Publisher John Wiley & Sons Inc
Language eng
Subject 0605 Microbiology
Abstract Cylindrospermopsis raciborskii is a bloom-forming cyanobacterium found in both tropical and temperate climates which produces cylindrospermopsin, a potent hepatotoxic secondary metabolite. This organism is notorious for its association with a significant human poisoning incident on Palm Island, Australia, which resulted in the hospitalization of 148 people. We have screened 13 C. raciborskii isolates from various regions of Australia and shown that both toxic and nontoxic strains exist within this species. No association was observed between geographical origin and toxin production. Polyketide synthases (PKSs) and peptide synthetases (PSs) are enzymes involved in secondary metabolite biosynthesis in cyanobacteria. Putative PKS and PS genes from C. raciborskii strains AWT205 and CYPO2OB were identified by PCR using degenerate primers based on conserved regions within each gene. Examination of the strain-specific distribution of the PKS and PS genes in C. raciborskii isolates demonstrated a direct link between the presence of these two genes and the ability to produce cylindrospermopsin. Interestingly, the possession of these two genes was also linked. They were also identified in an Anabaena bergii isolate that was demonstrated to produce cylindrospermopsin. Taken together, these data suggest a likely role for these determinants in secondary metabolite and toxin production by C. raciborskii. (C) 2001 John Wiley & Sons, Inc.
Keyword Environmental Sciences
Toxicology
Water Resources
Cylindrospermopsis Raciborskii
Cyanobacteria
Toxin Genes
Cylindrospermopsin
Nonribosomal Peptide-synthesis
Cyclic Heptapeptide Microcystin
Cultured Rat Hepatocytes
Synthetase Genes
Alkaloid Cylindrospermopsin
Secondary Metabolites
Polyketide Synthases
Umezakia Natans
Biosynthesis
Toxicity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Mon, 13 Aug 2007, 22:34:30 EST