Genetic Contexts of blaNDM-1 in Patients Carrying Multiple NDM-Producing Strains

Wailan, Alexander M., Sartor, Anna L., Zowawi, Hosam M., Perry, John D., Paterson, David L. and Sidjabat, Hanna E. (2015) Genetic Contexts of blaNDM-1 in Patients Carrying Multiple NDM-Producing Strains. Antimicrobial Agents and Chemotherapy, 59 12: 7405-7410. doi:10.1128/AAC.01319-15

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Author Wailan, Alexander M.
Sartor, Anna L.
Zowawi, Hosam M.
Perry, John D.
Paterson, David L.
Sidjabat, Hanna E.
Title Genetic Contexts of blaNDM-1 in Patients Carrying Multiple NDM-Producing Strains
Formatted title
Genetic Contexts of blaNDM-1 in Patients Carrying Multiple NDM-Producing Strains
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
1070-6283
Publication date 2015-09-21
Sub-type Article (original research)
DOI 10.1128/AAC.01319-15
Open Access Status File (Publisher version)
Volume 59
Issue 12
Start page 7405
End page 7410
Total pages 6
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The carbapenem resistance determinant blaNDM-1 has been found in various Gram-negative bacteria and upon different plasmid replicon types (Inc). Here, we present four patients within two hospitals in Pakistan harboring between two and four NDM-1-producing Gram-negative bacilli of different species coresident in their stool samples. We characterize the blaNDM-1 genetic contexts of these 11 NDM-1-producing Gram-negative bacilli in addition to other antimicrobial resistance mechanisms, plasmid replicon profiles, and sequence types (STs) in order to understand the underlying acquisition mechanisms of carbapenem resistance within these bacteria. Two common plasmid types (IncN2 and IncA/C) were identified to carry blaNDM-1 among the six different bacterial species isolated from the four patients. Two of these strains were novel Citrobacter freundii ST 20 and ST 21. The same IncN2-type blaNDM-1 genetic context was found in all four patients and within four different species. The IncA/C-type blaNDM-1 genetic context was found in two different species and in two of the four patients. Combining genetic context characterization with other molecular epidemiology methods, we were able to establish the molecular epidemiological links between genetically unrelated bacterial species by linking their acquisition of an IncN2 or IncA/C plasmid carrying blaNDM-1 for carbapenem resistance. By combining plasmid characterization and in-depth genetic context assessment, this analysis highlights the importance of plasmids in antimicrobial resistance. It also provides a novel approach for investigating the underlying mechanisms of blaNDM-1-related spread between bacterial species and genera via plasmids.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2016 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
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Created: Wed, 16 Dec 2015, 01:26:45 EST by Alexander Wailan on behalf of UQ Centre for Clinical Research