Genomic characteristics of NDM-producing Enterobacteriaceae in Australia and their blaNDM genetic contexts

Wailan, Alexander M., Paterson, David L., Kennedy, Karina, Ingram, Paul R., Bursle, Evan and Sidjabat, Hanna E. (2016) Genomic characteristics of NDM-producing Enterobacteriaceae in Australia and their blaNDM genetic contexts. Antimicrobial Agents and Chemotherapy, 60 1: 136-141. doi:10.1128/AAC.01243-15

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Author Wailan, Alexander M.
Paterson, David L.
Kennedy, Karina
Ingram, Paul R.
Bursle, Evan
Sidjabat, Hanna E.
Title Genomic characteristics of NDM-producing Enterobacteriaceae in Australia and their blaNDM genetic contexts
Formatted title
Genomic characteristics of NDM-producing Enterobacteriaceae in Australia and their blaNDM genetic contexts
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
1070-6283
Publication date 2016-01-01
Year available 2015
Sub-type Article (original research)
DOI 10.1128/AAC.01243-15
Open Access Status File (Publisher version)
Volume 60
Issue 1
Start page 136
End page 141
Total pages 24
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
blaNDM has been reported in different Enterobacteriaceae species and on numerous plasmid replicon types (Inc). Plasmid replicon typing, in combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer the spread of antimicrobial resistance determinants between genetically unrelated bacterial hosts. The genetic context of blaNDM is heterogeneous. In this study, we genomically characterized 12 NDM-producing Enterobacteriaceae isolated in Australia between 2012 and 2014: Escherichia coli (n = 6), Klebsiella pneumoniae (n = 3), Enterobacter cloacae (n = 2) and Providencia rettgeri (n = 1). We describe their blaNDM genetic contexts within Tn125, providing insights into the acquisition of blaNDM into Enterobacteriaceae. IncFII-type (n = 7) and IncX3 (n = 4) plasmids were the most common plasmid types found. The IncHI1B (n = 1) plasmid was also identified. Five different blaNDM genetic contexts were identified, indicating four particular plasmids with specific blaNDM genetic contexts (NGCs), three of which were IncFII plasmids (FII-A to -C). Of note, the blaNDM genetic context of P. rettgeri was not conjugative. Epidemiological links between our NDM-producing Enterobacteriaceae were established by their acquisition of these five particular plasmid types. The combination of different molecular and genetic characterization methods allowed us to provide insight into the spread of plasmids transmitting blaNDM.
Keyword Plasmids
blaNDM
IncFII
IncX3
Australia
Tn125
Genetic characterization
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2016 Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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Created: Wed, 16 Dec 2015, 01:18:47 EST by Alexander Wailan on behalf of UQ Centre for Clinical Research