APPL endosomes are not obligatory endocytic intermediates but act as stable cargo-sorting compartments

Kalaidzidis, Inna, Miaczynska, Marta, Brewinska-Olchowik, Marta, Hupalowska, Anna, Ferguson, Charles, Parton, Robert G., Kalaidzidis, Yannis and Zerial, Marino (2015) APPL endosomes are not obligatory endocytic intermediates but act as stable cargo-sorting compartments. Journal of Cell Biology, 211 1: 123-144. doi:10.1083/jcb.201311117

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Author Kalaidzidis, Inna
Miaczynska, Marta
Brewinska-Olchowik, Marta
Hupalowska, Anna
Ferguson, Charles
Parton, Robert G.
Kalaidzidis, Yannis
Zerial, Marino
Title APPL endosomes are not obligatory endocytic intermediates but act as stable cargo-sorting compartments
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
Publication date 2015-10-01
Year available 2015
Sub-type Article (original research)
DOI 10.1083/jcb.201311117
Open Access Status File (Publisher version)
Volume 211
Issue 1
Start page 123
End page 144
Total pages 22
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Subject 1307 Cell Biology
Abstract Endocytosis allows cargo to enter a series of specialized endosomal compartments, beginning with early endosomes harboring Rab5 and its effector EEA1. There are, however, additional structures labeled by the Rab5 effector APPL1 whose role in endocytic transport remains unclear. It has been proposed that APPL1 vesicles are transport intermediates that convert into EEA1 endosomes. Here, we tested this model by analyzing the ultrastructural morphology, kinetics of cargo transport, and stability of the APPL1 compartment over time. We found that APPL1 resides on a tubulo-vesicular compartment that is capable of sorting cargo for recycling or degradation and that displays long lifetimes, all features typical of early endosomes. Fitting mathematical models to experimental data rules out maturation of APPL1 vesicles into EEA1 endosomes as a primary mechanism for cargo transport. Our data suggest instead that APPL1 endosomes represent a distinct population of Rab5-positive sorting endosomes, thus providing important insights into the compartmental organization of the early endocytic pathway.
Keyword Clathrin-Mediated Endocytosis
Independent Endocytosis
Transferrin Receptor
Recycling Pathway
Coated Pits
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 511055
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
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Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
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