Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development

Liu, Yang, Xu, Xiao-Hui, Chen, Qi, Wang, Tong, Deng, Cai-Yun, Song, Bao-Liang, Du, Jiu-Lin and Luo, Zhen-Ge (2013) Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development. Nature Communications, 4 2005.1-2005.14. doi:10.1038/ncomms3005

Author Liu, Yang
Xu, Xiao-Hui
Chen, Qi
Wang, Tong
Deng, Cai-Yun
Song, Bao-Liang
Du, Jiu-Lin
Luo, Zhen-Ge
Title Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2013-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1038/ncomms3005
Open Access Status DOI
Volume 4
Start page 2005.1
End page 2005.14
Total pages 14
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Polarized membrane addition is crucial for axon development and elongation during neuronal morphogenesis. This process is believed to be regulated by directed membrane trafficking of Rab10-containing post-Golgi carriers. However, the mechanisms underlying the biogenesis of these carriers remain unclear. Here, we report that Rab10 interaction with myosin Vb (MYO5B) determines the formation of Rab10 carriers and is important for axon development. Rab10 interacts with the exon D-encoded domain of MYO5B. Downregulating the expression of MYO5B (+D) or blocking its interaction with Rab10 impairs the fission of Rab10 vesicles from trans-Golgi membranes, causes a decrease in the number of Rab10 transport carriers and inhibits axon development in cultured hippocampal neurons. Furthermore, the MYO5B-Rab10 system is required for axon development of vertebrate neocortical neurons or zebrafish retinal ganglion cells in vivo. Thus, specific interaction between Rab10 and MYO5B controls the formation of Rab10 vesicles, which is required for axon development.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 31021063
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 21 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 29 times in Scopus Article | Citations
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