MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development

Xu, Xiao-Hui, Deng, Cai-Yun, Liu, Yang, He, Miao, Peng, Jian, Wang, Tong, Yuan, Lei, Zheng, Zhi-Sheng, Blackshear, Perry J. and Luo, Zhen-Ge (2014) MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development. Cell Research, 24 5: 576-594. doi:10.1038/cr.2014.33

Author Xu, Xiao-Hui
Deng, Cai-Yun
Liu, Yang
He, Miao
Peng, Jian
Wang, Tong
Yuan, Lei
Zheng, Zhi-Sheng
Blackshear, Perry J.
Luo, Zhen-Ge
Title MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development
Journal name Cell Research   Check publisher's open access policy
ISSN 1748-7838
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1038/cr.2014.33
Open Access Status Not Open Access
Volume 24
Issue 5
Start page 576
End page 594
Total pages 19
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Axon development requires membrane addition from the intracellular supply, which has been shown to be mediated by Rab10-positive plasmalemmal precursor vesicles (PPVs). However, the molecular mechanisms underlying the membrane trafficking processes of PPVs remain unclear. Here, we show that myristoylated alanine-rich C-kinase substrate (MARCKS) mediates membrane targeting of Rab10-positive PPVs, and this regulation is critical for axon development. We found that the GTP-locked active form of Rab10 binds to membrane-associated MARCKS, whose affinity depends on the phosphorylation status of the MARCKS effector domain. Either genetic silencing of MARCKS or disruption of its interaction with Rab10 inhibited axon growth of cortical neurons, impaired docking and fusion of Rab10 vesicles with the plasma membrane, and consequently caused a loss of membrane insertion of axonal receptors responsive to extracellular axon growth factors. Thus, this study has identified a novel function of MARCKS in mediating membrane targeting of PPVs during axon development.
Keyword Axon growth
Plasmalemmal precursor vesicles
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 31330032
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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