Interleukin-10 and interleukin-18 promoter polymorphisms in an Italian cohort of patients with undifferentiated carcinoma of nasopharyngeal type

Pratesi, Chiara, Bortolin, Maria Teresa, Bidoli, Ettore, Tedeschi, Rosamaria, Vaccher, Emanuela, Dolcetti, Riccardo, Guidoboni, Massimo, Franchin, Gianni, Barzan, Luigi, Zanussi, Stefania, Caruso, Calogero and De Paoli, Paolo (2006) Interleukin-10 and interleukin-18 promoter polymorphisms in an Italian cohort of patients with undifferentiated carcinoma of nasopharyngeal type. Cancer Immunology, Immunotherapy, 55 1: 23-30. doi:10.1007/s00262-005-0688-z


Author Pratesi, Chiara
Bortolin, Maria Teresa
Bidoli, Ettore
Tedeschi, Rosamaria
Vaccher, Emanuela
Dolcetti, Riccardo
Guidoboni, Massimo
Franchin, Gianni
Barzan, Luigi
Zanussi, Stefania
Caruso, Calogero
De Paoli, Paolo
Title Interleukin-10 and interleukin-18 promoter polymorphisms in an Italian cohort of patients with undifferentiated carcinoma of nasopharyngeal type
Journal name Cancer Immunology, Immunotherapy   Check publisher's open access policy
ISSN 0340-7004
1432-0851
Publication date 2006-01-01
Year available 2005
Sub-type Article (original research)
DOI 10.1007/s00262-005-0688-z
Open Access Status Not yet assessed
Volume 55
Issue 1
Start page 23
End page 30
Total pages 8
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Purpose: Cytokines such as IL-10 and IL-18 seem to be involved in the inflammatory response of undifferentiated carcinoma of nasopharyngeal type (UCNT). The aim of this study was to evaluate the correlation between functional single nucleotide polymorphisms (SNPs) in the promoter region of IL-10 and IL-18 genes and the virological and clinical characteristics in a large case series of Caucasian patients suffering from UCNT, a tumor regularly associated with the Epstein Barr Virus (EBV).

Methods: Eighty-nine patients with histologically confirmed UCNT and 130 healthy donors were included in our study. DNA was examined for the polymorphisms of IL-10 gene at positions -1082, -819, -592 by direct sequencing and IL-18 gene at position -607 and -137 by allele -specific PCR. EBV DNA serum viremia was evaluated by QC-PCR.

Results: The distributions of the IL-10 and IL-18 genetic variants were not different between UCNT patients and healthy controls. The frequency of IL-10 - 1082G allele, which is associated with high IL-10 expression, showed a nearly statistically significant increase in UCNT patients EBV DNA-negative as compared to healthy controls (OR = 3.3 95% CI: 1.2-9.8). Subjects with C/C or C/G combined IL-18 genotypes showed an increased risk of being with Stages III-IV (OR = 2.1 95% CI: 1.2-6.6).

Conclusion: This study was performed to improve the definition of the pathogenetic factors implicated in UCNT by addressing the correlation between cytokine polymorphisms and clinical parameters. This is the first study investigating the possible role of the IL-18 and IL-10 polymorphisms in the development and outcome of UCNT. In our genetic analysis there is no evidence for involvement of IL-10 promoter polymorphisms alone in the genetic predisposition to this tumor. On the other hand, IL18 genetic variants may represent a genetic risk factor for tumor aggressiveness.
Keyword Epstein Barr virus (EBV)
Interleukin-10
Interleukin-18
Single nucleotide polymorphisms (SNPs)
Undifferentiated carcinoma of nasopharyngeal type (UCNT)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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