Utility of Helicobacter spp. associated GFD markers for detecting avian fecal pollution in natural waters of two continents

Ahmed, W., Harwood, V. J., Nguyen, K., Young, S., Hamilton, K. and Toze, S. (2016) Utility of Helicobacter spp. associated GFD markers for detecting avian fecal pollution in natural waters of two continents. Water Research, 88 613-622. doi:10.1016/j.watres.2015.10.050


Author Ahmed, W.
Harwood, V. J.
Nguyen, K.
Young, S.
Hamilton, K.
Toze, S.
Title Utility of Helicobacter spp. associated GFD markers for detecting avian fecal pollution in natural waters of two continents
Formatted title
Utility of Helicobacter spp. associated GFD markers for detecting avian fecal pollution in natural waters of two continents
Journal name Water Research   Check publisher's open access policy
ISSN 1879-2448
0043-1354
Publication date 2016-01-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.watres.2015.10.050
Open Access Status Not Open Access
Volume 88
Start page 613
End page 622
Total pages 10
Place of publication London, United Kingdom
Publisher IWA Publishing
Language eng
Formatted abstract
Avian fecal droppings may negatively impact environmental water quality due to the presence of high concentrations of fecal indicator bacteria (FIB) and zoonotic pathogens. This study was aimed at evaluating the performance characteristics and utility of a Helicobacter spp. associated GFD marker by screening 265 fecal and wastewater samples from a range of avian and non-avian host groups from two continents (Brisbane, Australia and Florida, USA). The host-prevalence and -specificity of this marker among fecal and wastewater samples tested from Brisbane were 0.58 and 0.94 (maximum value of 1.00). These values for the Florida fecal samples were 0.30 (host-prevalence) and 1.00 (host-specificity). The concentrations of the GFD markers in avian and non-avian fecal nucleic acid samples were measured at a test concentration of 10 ng of nucleic acid at Brisbane and Florida laboratories using the quantitative PCR (qPCR) assay. The mean concentrations of the GFD marker in avian fecal nucleic acid samples (5.2 × 103 gene copies) were two orders of magnitude higher than non-avian fecal nucleic acid samples (8.6 × 101 gene copies). The utility of this marker was evaluated by testing water samples from the Brisbane River, Brisbane and a freshwater creek in Florida. Among the 18 water samples tested from the Brisbane River, 83% (n = 18) were positive for the GFD marker, and the concentrations ranged from 6.0 × 101–3.2 × 102 gene copies per 100 mL water. In all, 92% (n = 25) water samples from the freshwater creek in Florida were also positive for the GFD marker with concentrations ranging from 2.8 × 101–1.3 × 104 gene copies per 100 mL water. Based on the results, it can be concluded that the GFD marker is highly specific to avian host groups, and could be used as a reliable marker to detect the presence and amount of avian fecal pollution in environmental waters.
Keyword Microbial source tracking
Fecal indicator bacteria
Avian fecal pollution
Molecular markers
Wastewater
Quantitative PCR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Public Health Publications
 
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