BCG skin infection triggers IL-1R-MyD88-dependent migration of EpCAMlow CD11bhigh skin dendritic cells to draining lymph node during CD4+ T-cell priming

Bollampalli, Vishnu P., Harumi Yamashiro, Livia, Feng, Xiaogang, Bierschenk, Damien, Gao, Yu, Blom, Hans, Henriques-Normark, Birgitta, Nylen, Susanne and Rothfuchs, Antonio Gigliotti (2015) BCG skin infection triggers IL-1R-MyD88-dependent migration of EpCAMlow CD11bhigh skin dendritic cells to draining lymph node during CD4+ T-cell priming. PLoS Pathogens, 11 10: 1-22. doi:10.1371/journal.ppat.1005206


Author Bollampalli, Vishnu P.
Harumi Yamashiro, Livia
Feng, Xiaogang
Bierschenk, Damien
Gao, Yu
Blom, Hans
Henriques-Normark, Birgitta
Nylen, Susanne
Rothfuchs, Antonio Gigliotti
Title BCG skin infection triggers IL-1R-MyD88-dependent migration of EpCAMlow CD11bhigh skin dendritic cells to draining lymph node during CD4+ T-cell priming
Formatted title
BCG skin infection triggers IL-1R-MyD88-dependent migration of EpCAMlow CD11bhigh skin dendritic cells to draining lymph node during CD4+ T-cell priming
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7374
Publication date 2015-10-06
Year available 2015
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1005206
Open Access Status DOI
Volume 11
Issue 10
Start page 1
End page 22
Total pages 22
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Formatted abstract
The transport of antigen from the periphery to the draining lymph node (DLN) is critical for T-cell priming but remains poorly studied during infection with Mycobacterium bovis Bacille Calmette-Guérin (BCG). To address this we employed a mouse model to track the traffic of Dendritic cells (DCs) and mycobacteria from the BCG inoculation site in the skin to the DLN. Detection of BCG in the DLN was concomitant with the priming of antigen-specific CD4+ T cells at that site. We found EpCAMlow CD11bhigh migratory skin DCs to be mobilized during the transport of BCG to the DLN. Migratory skin DCs distributed to the T-cell area of the LN, co-localized with BCG and were found in close apposition to antigen-specific CD4+ T cells. Consequently, blockade of skin DC traffic into DLN dramatically reduced mycobacterial entry into DLN and muted T-cell priming. Interestingly, DC and mycobacterial entry into the DLN was dependent on IL-1R-I, MyD88, TNFR-I and IL-12p40. In addition, we found using DC adoptive transfers that the requirement for MyD88 in BCG-triggered migration was not restricted to the migrating DC itself and that hematopoietic expression of MyD88 was needed in part for full-fledged migration. Our observations thus identify a population of DCs that contribute towards the priming of CD4+ T cells to BCG infection by transporting bacilli into the DLN in an IL-1R-MyD88-dependent manner and reveal both DC-intrinsic and -extrinsic requirements for MyD88 in DC migration.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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