Synthesis of silica nanoparticles with controllable surface roughness for therapeutic protein delivery

Niu, Yuting, Yu, Meihua, Zhang, Jun, Yang, Yannan, Xu, Chun, Yeh, Michael, Taran, Elena, Hou, Jeff Jia Cheng, Gray, Peter P. and Yu, Chengzhong (2015) Synthesis of silica nanoparticles with controllable surface roughness for therapeutic protein delivery. Journal of Materials Chemistry B, 3 43: 8477-8485. doi:10.1039/c5tb01405k


Author Niu, Yuting
Yu, Meihua
Zhang, Jun
Yang, Yannan
Xu, Chun
Yeh, Michael
Taran, Elena
Hou, Jeff Jia Cheng
Gray, Peter P.
Yu, Chengzhong
Title Synthesis of silica nanoparticles with controllable surface roughness for therapeutic protein delivery
Journal name Journal of Materials Chemistry B   Check publisher's open access policy
ISSN 2050-750X
2050-7518
Publication date 2015-09-16
Sub-type Article (original research)
DOI 10.1039/c5tb01405k
Open Access Status Not Open Access
Volume 3
Issue 43
Start page 8477
End page 8485
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2016
Language eng
Abstract There is an increasing demand of efficient nano-carriers for intracellular delivery of therapeutic proteins. This study reports on a novel “neck-enhancing” approach to synthesize stable rough silica nanoparticles (RSNs) with controllable surface roughness. By increasing the shell particle size from 13 to 98 nm while fixing the core size at 211 nm, the interspace size between neighboring shell particles of RSNs is enlarged from 7 to 38 nm. Cytochrome c, IgG fragment and IgG antibody are preferably adsorbed onto one of the RSNs with the interspace size of 14, 21 and 38 nm, respectively. The binding activity of the IgG fragment loaded onto RSNs is maintained as confirmed by surface plasmon resonance. The hydrophobically modified RSN with the interspace size of 38 nm effectively deliver the therapeutic anti-pAkt antibody into breast cancer cells, causing significant cell inhibition by blocking pAkt and the downstream anti-apoptotic protein Bcl-2.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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