Estradiol-regulated expression of the immunophilins cyclophilin 40 and FKBP52 in MCF-7 breast cancer cells

Kumar, P, Mark, PJ, Ward, BK, Minchin, RF and Ratajczak, T (2001) Estradiol-regulated expression of the immunophilins cyclophilin 40 and FKBP52 in MCF-7 breast cancer cells. Biochemical And Biophysical Research Communications, 284 1: 219-225. doi:10.1006/bbrc.2001.4952


Author Kumar, P
Mark, PJ
Ward, BK
Minchin, RF
Ratajczak, T
Title Estradiol-regulated expression of the immunophilins cyclophilin 40 and FKBP52 in MCF-7 breast cancer cells
Journal name Biochemical And Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2001-01-01
Sub-type Article (original research)
DOI 10.1006/bbrc.2001.4952
Open Access Status Not Open Access
Volume 284
Issue 1
Start page 219
End page 225
Total pages 7
Place of publication San Diego
Publisher Academic Press Inc
Language eng
Abstract The immunophilins, cyclophilin 40 (CyP40) and FKBP52, are associated with the unactivated estrogen receptor in mutually exclusive heterocomplexes and may differentially modulate receptor activity, We have recently shown that CyP40 and FKBP52 mRNA's are differentially elevated in breast carcinomas compared with normal breast tissue. Other studies suggest that such alterations ill the ratio of immunophilins might potentially influence steroid receptor function. Studies were therefore initiated to investigate the influence of estradiol on CyP40 and FKBP52 expression in MCF-7 breast cancer cells. Over a 24-h-treatment period with estradiol, CyP40 and FKBP52 mRNA expression was increased approximately five- and 14-fold, respectively. The corresponding protein levels were also elevated in comparison to controls. The antiestrogen, ICI 182,780, was an antagonist for CyP40 and FKBP52 mRNA induction. Cycloheximide treatment did not inhibit this increased immunophilin expression, suggesting that estradiol-mediated activation is independent off de novo protein synthesis. Treatment of MCF-7 cells with estradiol resulted in an increased half-life of both CyP40 and FKBP52 mRNA, as determined by actinomycin D studies. These results suggest that estradiol regulates CyP40 and FKBP52 mRNA expression through both transcriptional and posttranscriptional mechanisms. (C) 2001 Academic Press.
Keyword Biochemistry & Molecular Biology
Biophysics
Cyclophilin 40
Fkbp52
Protein Expression
Mrna Expression
Mrna Stability
Estrogen Receptor
Estradiol
Heat-shock-protein
Estrogen-receptor
Progesterone-receptor
Messenger-rna
Hsp90
Growth
Complexes
Component
Mutants
Alpha
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Mon, 13 Aug 2007, 22:22:05 EST