AllR controls the expression of Streptomyces coelicolor allantoin pathway genes

Navone, Laura, Pablo Macagno, Juan, Licona-Cassani, Cuauhtemoc, Marcellin, Esteban, Nielsen, Lars K., Gramajo, Hugo and Rodriguez, Eduardo (2015) AllR controls the expression of Streptomyces coelicolor allantoin pathway genes. Applied and Environmental Microbiology, 81 19: 6649-6659. doi:10.1128/AEM.02098-15

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Author Navone, Laura
Pablo Macagno, Juan
Licona-Cassani, Cuauhtemoc
Marcellin, Esteban
Nielsen, Lars K.
Gramajo, Hugo
Rodriguez, Eduardo
Title AllR controls the expression of Streptomyces coelicolor allantoin pathway genes
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 1098-5336
Publication date 2015-01-01
Sub-type Article (original research)
DOI 10.1128/AEM.02098-15
Open Access Status File (Publisher version)
Volume 81
Issue 19
Start page 6649
End page 6659
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Streptomyces species are native inhabitants of soil, a natural environment where nutrients can be scarce and competition fierce. They have evolved ways to metabolize unusual nutrients, such as purines and its derivatives, which are highly abundant in soil. Catabolism of these uncommon carbon and nitrogen sources needs to be tightly regulated in response to nutrient availability and environmental stimulus. Recently, the allantoin degradation pathway was characterized in Streptomyces coelicolor. However, there are questions that remained unanswered, particularly regarding pathway regulation. Here, using a combination of proteomics and genetic approaches, we identified the negative regulator of the allantoin pathway, AllR. In vitro studies confirmed that AllR binds to the promoter regions of allantoin catabolic genes and determined the AllR DNA binding motif. In addition, effector studies showed that allantoic acid, and glyoxylate, to a lesser extent, inhibit the binding of AllR to the DNA. Inactivation of AllR repressor leads to the constitutive expression of the AllR regulated genes and intriguingly impairs actinorhodin and undecylprodigiosin production. Genetics and proteomics analysis revealed that among all genes from the allantoin pathway that are upregulated in the allR mutant, the hyi gene encoding a hydroxypyruvate isomerase (Hyi) is responsible of the impairment of antibiotic production.
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Document type: Journal Article
Sub-type: Article (original research)
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Australian Institute for Bioengineering and Nanotechnology Publications
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