Histopathological studies of the process of Phytophthora palmivora infection in oil palm

Sarria, G. A., Martinez, G., Varon, F., Drenth, A. and Guest, D. I. (2015) Histopathological studies of the process of Phytophthora palmivora infection in oil palm. European Journal of Plant Pathology, 145 1: 1-13. doi:10.1007/s10658-015-0810-9


Author Sarria, G. A.
Martinez, G.
Varon, F.
Drenth, A.
Guest, D. I.
Title Histopathological studies of the process of Phytophthora palmivora infection in oil palm
Journal name European Journal of Plant Pathology   Check publisher's open access policy
ISSN 0929-1873
1573-8469
Publication date 2015-10-28
Year available 2016
Sub-type Article (original research)
DOI 10.1007/s10658-015-0810-9
Open Access Status Not Open Access
Volume 145
Issue 1
Start page 1
End page 13
Total pages 13
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Subject 1102 Agronomy and Crop Science
1110 Plant Science
1108 Horticulture
Abstract The predominant disease affecting oil palms in Colombia is bud rot, a decay of the growing leaf tissues that may eventually reach the meristem. Previous studies have suggested many biotic and abiotic causes, but Koch’s Postulates have recently confirmed Phytophthora palmivora as the primary pathogen of bud rot. In this paper, we describe the infection process in immature leaflets inoculated with zoospores of P. palmivora using light microscopy and scanning electron microscopy. Initially, zoospores were attracted primarily to leaf trichomes, where some developed appressorium-like structures and subsequently penetrated the tissue. Germinated zoospores were also observed penetrating the stomata or intercellular spaces. After penetration hyphae colonized the intercellular leaf spaces, followed by intracellular invasion, penetrating to the vascular bundles and then spreading throughout the host tissue. Between 12 and 24 h after inoculation, hyphae emerged from the surface of the leaflet through the stomata, trichomes and intercellular spaces. Within 36 and 48 h, sporangia, chlamydospores, and newly released zoospores were observed, starting a new cycle of secondary infections. This sequence was consistently observed in repeated experiments. We suggest that in the field collection of moisture at the base of emerging leaflets stimulates the release of zoospores and infection. Colonization is restricted once the leaflet emerges, leaving dormant chlamydospores and hyphae in small necrotic lesions. Successive rain events stimulate the formation of new generations of sporangia, zoospores and infections, which, if frequent enough, may completely colonize the bud area. Subsequent colonization of infected tissues by opportunistic bacteria and fungi increases the severity of bud rot symptoms. The information presented here provides an overview of histopathological aspects of the early infections process of P. palmivora in detached immature leaflets.
Keyword E. guineensis
Infection
PC
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2016 Collection
 
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