An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine

Parker, Suzanne L., Adnan, Syamhanin, Meija, Jenny L. Ordonez, Paterson, David L., Lipman, Jeffrey, Roberts, Jason A. and Wallis, Steven C. (2015) An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine. Bioanalysis, 7 18: 2311-2319. doi:10.4155/bio.15.143


Author Parker, Suzanne L.
Adnan, Syamhanin
Meija, Jenny L. Ordonez
Paterson, David L.
Lipman, Jeffrey
Roberts, Jason A.
Wallis, Steven C.
Title An UHPLC-MS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma and urine
Journal name Bioanalysis   Check publisher's open access policy
ISSN 1757-6199
1757-6180
Publication date 2015-09-01
Year available 2015
Sub-type Article (original research)
DOI 10.4155/bio.15.143
Open Access Status Not yet assessed
Volume 7
Issue 18
Start page 2311
End page 2319
Total pages 9
Place of publication London, United Kingdom
Publisher Future Science
Language eng
Formatted abstract
Background: With few new antibiotics available and an increased prevalence of multidrug-resistant bacteria, optimizing the effectiveness of currently available antibiotics and minimizing the potential for emerging resistance, through PK characterization, is of paramount importance. Results: Inter- and intra-assay results for ampicillin were within 5% for accuracy and 7% for precision, and for sulbactam within 7% for accuracy and 7% for precision, for both plasma and urine. The plasma Cmax was 38.4 µg/ml for ampicillin and 19.0 µg/ml for sulbactam. The urine concentrations were 668 µg/ml for ampicillin and 438 µg/ml for sulbactam. Conclusion: An UHPLC–MS/MS method to simultaneously measure co-formulated ampicillin and sulbactam in plasma and urine, for use in a patient PK study, has been developed and validated.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2016 Collection
School of Medicine Publications
 
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