Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca2+ sensor

Guo, Zhong, Johnston, Wayne A., Stein, Viktor, Kalimuthu, Palraj, Perez-Alcala, Siro, Bernhardt, Paul V. and Alexandrov, Kirill (2015) Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca2+ sensor. Chemical Communications, 52 3: 1-4. doi:10.1039/C5CC07824E


Author Guo, Zhong
Johnston, Wayne A.
Stein, Viktor
Kalimuthu, Palraj
Perez-Alcala, Siro
Bernhardt, Paul V.
Alexandrov, Kirill
Title Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca2+ sensor
Journal name Chemical Communications   Check publisher's open access policy
ISSN 1364-548X
1359-7345
Publication date 2015-11-03
Sub-type Article (original research)
DOI 10.1039/C5CC07824E
Open Access Status Not Open Access
Volume 52
Issue 3
Start page 1
End page 4
Total pages 4
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
Electrochemical biosensors convert biological events to an electrical current. To date most electrochemical biosensors exploit activities of naturally occurring enzymes. Here we demonstrated that insertion of a calmodulin domain into the redox enzyme PQQ-glucose dehydrogenase resulted in a selective Ca2+ biosensor that could be used to rapidly measure Ca2+ concentrations in human biological fluids. We were able to convert a point-of-care glucometer into Ca2+ monitor by refurbishing it with the developed biosensor. We propose that similar engineering strategies may be used to create highly specific electrochemical biosensors to other analytes. Compatibility with cheap and ubiquitous amperometric detectors is expected to accelerate progression of these biosensors into clinical applications.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 06 Nov 2015, 19:35:36 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences