A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia

McLean, Breeanna J., Hobson-Peters, Jody, Webb, Cameron E., Watterson, Daniel, Prow, Natalie A., Nguyen, Hong Duyen, Hall-Mendelin, Sonja, Warrilow, David, Johansen, Cheryl A., Jansen, Cassie C., van den Hurk, Andrew F., Beebe, Nigel W., Schnettler, Esther, Barnard, Ross T. and Hall, Roy A. (2015) A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia. Virology, 486 272-283. doi:10.1016/j.virol.2015.07.021


Author McLean, Breeanna J.
Hobson-Peters, Jody
Webb, Cameron E.
Watterson, Daniel
Prow, Natalie A.
Nguyen, Hong Duyen
Hall-Mendelin, Sonja
Warrilow, David
Johansen, Cheryl A.
Jansen, Cassie C.
van den Hurk, Andrew F.
Beebe, Nigel W.
Schnettler, Esther
Barnard, Ross T.
Hall, Roy A.
Title A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia
Formatted title
A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1096-0341
Publication date 2015-10-27
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.virol.2015.07.021
Open Access Status Not Open Access
Volume 486
Start page 272
End page 283
Total pages 12
Place of publication Waltham, MA, United States
Publisher Academic Press
Language eng
Subject 2406 Virology
2700 Medicine
Abstract To date, insect-specific flaviviruses (ISFs) have only been isolated from mosquitoes and increasing evidence suggests that ISFs may affect the transmission of pathogenic flaviviruses. To investigate the diversity and prevalence of ISFs in Australian mosquitoes, samples from various regions were screened for flaviviruses by ELISA and RT-PCR. Thirty-eight pools of Aedes vigilax from Sydney in 2007 yielded isolates of a novel flavivirus, named Parramatta River virus (PaRV). Sequencing of the viral RNA genome revealed it was closely related to Hanko virus with 62.3% nucleotide identity over the open reading frame. PaRV failed to grow in vertebrate cells, with only Aedes-derived mosquito cell lines permissive to replication, suggesting a narrow host range. 2014 collections revealed that PaRV had persisted in A. vigilax populations in Sydney, with 88% of pools positive. Further investigations into its mode of transmission and potential to influence vector competence of A. vigilax for pathogenic viruses are warranted.
Formatted abstract
To date, insect-specific flaviviruses (ISFs) have only been isolated from mosquitoes and increasing evidence suggests that ISFs may affect the transmission of pathogenic flaviviruses. To investigate the diversity and prevalence of ISFs in Australian mosquitoes, samples from various regions were screened for flaviviruses by ELISA and RT-PCR. Thirty-eight pools of Aedes vigilax from Sydney in 2007 yielded isolates of a novel flavivirus, named Parramatta River virus (PaRV). Sequencing of the viral RNA genome revealed it was closely related to Hanko virus with 62.3% nucleotide identity over the open reading frame. PaRV failed to grow in vertebrate cells, with only Aedes-derived mosquito cell lines permissive to replication, suggesting a narrow host range. 2014 collections revealed that PaRV had persisted in A. vigilax populations in Sydney, with 88% of pools positive. Further investigations into its mode of transmission and potential to influence vector competence of A. vigilax for pathogenic viruses are warranted.
Keyword Aedes vigilax
Arbovirus
Coquillettidia xanthogaster
Insect-specific flavivirus
Mosquito
Palm Creek virus
Parramatta River virus
Virus discovery
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP120103994
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 06 Nov 2015, 19:25:56 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences