Dengue virus infection alters posttranscriptional modification of microRNAs in the mosquito vector Aedes aegypti

Etebari, Kayvan, Osei-Amo, Solomon, Blomberg, Simon Phillip and Asgari, Sassan (2015) Dengue virus infection alters posttranscriptional modification of microRNAs in the mosquito vector Aedes aegypti. Scientific Reports, 5 15968.1-15968.14. doi:10.1038/srep15968

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Author Etebari, Kayvan
Osei-Amo, Solomon
Blomberg, Simon Phillip
Asgari, Sassan
Title Dengue virus infection alters posttranscriptional modification of microRNAs in the mosquito vector Aedes aegypti
Formatted title
Dengue virus infection alters posttranscriptional modification of microRNAs in the mosquito vector Aedes aegypti
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-10-30
Sub-type Article (original research)
DOI 10.1038/srep15968
Open Access Status DOI
Volume 5
Start page 15968.1
End page 15968.14
Total pages 14
Place of publication London, United Kingdom
Publisher Nature Publishing
Collection year 2016
Language eng
Formatted abstract
Recent discoveries regarding the importance of isomiRs have increased our understanding of the regulatory complexities of the miRNAome. Observed changes in the miRNA profiles in mosquitoes infected with flaviviruses have implicated small RNAs in the interactions between viruses and their vectors. Here we analysed the isomiR profiles of both uninfected and infected Aedes aegypti mosquitoes with the major human pathogen dengue virus (DENV). We found that several specific isomiRs were significantly altered in their abundance patterns in response to DENV infection potentially affecting their target repertoire. Notable among these were isomiR variants which displayed arm-switching. We also demonstrate that modifications to the 3p end of miRNAs are vastly more prevalent than those at the 5p ends. We also observed that in only 45% of Ae. aegypti miRNAs the most abundant read matches the exact sequence reported in miRBase. Further, we found positive correlations between the number of mature miRNA reads, pre-miRNA length, GC content and secondary structure minimum free energy with the number of isomiRs. The findings presented here provide some evidence that isomiR production is not a random phenomenon and may be important in DENV replication in its vector.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
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Created: Mon, 02 Nov 2015, 06:37:42 EST by Prof Sassan Asgari on behalf of School of Biological Sciences