Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing

Jaenicke, Sebastian, Ander, Christina, Bekel, Thomas, Bisdorf, Regina, Droege, Marcus, Gartemann, Karl-Heinz, Juenemann, Sebastian, Kaiser, Olaf, Krause, Lutz, Tille, Felix, Zakrzewski, Martha, Puehler, Alfred, Schlueter, Andreas and Goesmann, Alexander (2011) Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing. PLoS ONE, 6 1: . doi:10.1371/journal.pone.0014519

Author Jaenicke, Sebastian
Ander, Christina
Bekel, Thomas
Bisdorf, Regina
Droege, Marcus
Gartemann, Karl-Heinz
Juenemann, Sebastian
Kaiser, Olaf
Krause, Lutz
Tille, Felix
Zakrzewski, Martha
Puehler, Alfred
Schlueter, Andreas
Goesmann, Alexander
Title Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2011-01-26
Year available 2011
Sub-type Article (original research)
DOI 10.1371/journal.pone.0014519
Open Access Status DOI
Volume 6
Issue 1
Total pages 15
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract Biogas production from renewable resources is attracting increased attention as an alternative energy source due to the limited availability of traditional fossil fuels. Many countries are promoting the use of alternative energy sources for sustainable energy production. In this study, a metagenome from a production-scale biogas fermenter was analysed employing Roche's GS FLX Titanium technology and compared to a previous dataset obtained from the same community DNA sample that was sequenced on the GS FLX platform. Taxonomic profiling based on 16S rRNA-specific sequences and an Environmental Gene Tag (EGT) analysis employing CARMA demonstrated that both approaches benefit from the longer read lengths obtained on the Titanium platform. Results confirmed Clostridia as the most prevalent taxonomic class, whereas species of the order Methanomicrobiales are dominant among methanogenic Archaea. However, the analyses also identified additional taxa that were missed by the previous study, including members of the genera Streptococcus, Acetivibrio, Garciella, Tissierella, and Gelria, which might also play a role in the fermentation process leading to the formation of methane. Taking advantage of the CARMA feature to correlate taxonomic information of sequences with their assigned functions, it appeared that Firmicutes, followed by Bacteroidetes and Proteobacteria, dominate within the functional context of polysaccharide degradation whereas Methanomicrobiales represent the most abundant taxonomic group responsible for methane production. Clostridia is the most important class involved in the reductive CoA pathway (Wood-Ljungdahl pathway) that is characteristic for acetogenesis. Based on binning of 16S rRNA-specific sequences allocated to the dominant genus Methanoculleus, it could be shown that this genus is represented by several different species. Phylogenetic analysis of these sequences placed them in close proximity to the hydrogenotrophic methanogen Methanoculleus bourgensis. While rarefaction analyses still indicate incomplete coverage, examination of the GS FLX Titanium dataset resulted in the identification of additional genera and functional elements, providing a far more complete coverage of the community involved in anaerobic fermentative pathways leading to methane formation.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 0313704
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 113 times in Thomson Reuters Web of Science Article | Citations
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