The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology

Schlueter, Andreas, Bekel, Thomas, Diaz, Naryttza N., Dondrup, Michael, Eichenlaub, Rudolf, Gartemann, Karl-Heinz, Krahn, Irene, Krause, Lutz, Kroemeke, Holger, Kruse, Olaf, Mussgnug, Jan H., Neuweger, Heiko, Niehaus, Karsten, Puehler, Alfred, Runte, Kai J., Szczepanowski, Rafael, Tauch, Andreas, Tilker, Alexandra, Viehoever, Prisca and Goesmann, Alexander (2008) The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology. Journal of Biotechnology, 136 1-2: 77-90. doi:10.1016/j.jbiotec.2008.05.008


Author Schlueter, Andreas
Bekel, Thomas
Diaz, Naryttza N.
Dondrup, Michael
Eichenlaub, Rudolf
Gartemann, Karl-Heinz
Krahn, Irene
Krause, Lutz
Kroemeke, Holger
Kruse, Olaf
Mussgnug, Jan H.
Neuweger, Heiko
Niehaus, Karsten
Puehler, Alfred
Runte, Kai J.
Szczepanowski, Rafael
Tauch, Andreas
Tilker, Alexandra
Viehoever, Prisca
Goesmann, Alexander
Title The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology
Journal name Journal of Biotechnology   Check publisher's open access policy
ISSN 0168-1656
1873-4863
Publication date 2008-08-31
Sub-type Article (original research)
DOI 10.1016/j.jbiotec.2008.05.008
Open Access Status Not yet assessed
Volume 136
Issue 1-2
Start page 77
End page 90
Total pages 14
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
Composition and gene content of a biogas-producing microbial community from a production-scale biogas plant fed with renewable primary products was analysed by means of a metagenomic approach applying the ultrafast 454-pyrosequencing technology. Sequencing of isolated total community DNA on a Genome Sequencer FLX System resulted in 616,072 reads with an average read length of 230 bases accounting for 141,664,289 bases sequence information. Assignment of obtained single reads to COG (Clusters of Orthologous Groups of proteins) categories revealed a genetic profile characteristic for an anaerobic microbial consortium conducting fermentative metabolic pathways. Assembly of single reads resulted in the formation of 8752 contigs larger than 500 bases in size. Contigs longer than 10 kb mainly encode house-keeping proteins, e.g. DNA polymerase, recombinase, DNA ligase, sigma factor RpoD and genes involved in sugar and amino acid metabolism. A significant portion of contigs was allocated to the genome sequence of the archaeal methanogen Methanoculleus marisnigri JR1. Mapping of single reads to the M. marisnigri JR1 genome revealed that approximately 64% of the reference genome including methanogenesis gene regions are deeply covered. These results suggest that species related to those of the genus Methanoculleus play a dominant role in methanogenesis in the analysed fermentation sample. Moreover, assignment of numerous contig sequences to clostridial genomes including gene regions for cellulolytic functions indicates that clostridia are important for hydrolysis of cellulosic plant biomass in the biogas fermenter under study. Metagenome sequence data from a biogas-producing microbial community residing in a fermenter of a biogas plant provide the basis for a rational approach to improve the biotechnological process of biogas production.
Keyword 454-Pyrosequencing
Biogas fermentation
Metagenome
Methane production
Methanogenesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 166 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 180 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 31 Oct 2015, 05:16:24 EST by System User on behalf of Learning and Research Services (UQ Library)