Safety analysis of a Russian phage cocktail: from MetaGenomic analysis to oral application in healthy human subjects

McCallin, Shawna, Sarker, Shafiqul Alam, Barretto, Caroline, Sultana, Shamima, Berger, Bernard, Huq, Sayeda, Krause, Lutz, Bibiloni, Rodrigo, Schmitt, Bertrand, Reuteler, Gloria and Bruessow, Harald (2013) Safety analysis of a Russian phage cocktail: from MetaGenomic analysis to oral application in healthy human subjects. Virology, 443 2: 187-196. doi:10.1016/j.virol.2013.05.022


Author McCallin, Shawna
Sarker, Shafiqul Alam
Barretto, Caroline
Sultana, Shamima
Berger, Bernard
Huq, Sayeda
Krause, Lutz
Bibiloni, Rodrigo
Schmitt, Bertrand
Reuteler, Gloria
Bruessow, Harald
Title Safety analysis of a Russian phage cocktail: from MetaGenomic analysis to oral application in healthy human subjects
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1096-0341
1089-862X
Publication date 2013-09-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.virol.2013.05.022
Open Access Status DOI
Volume 443
Issue 2
Start page 187
End page 196
Total pages 10
Place of publication Waltham, MA, United States
Publisher Academic Press
Language eng
Formatted abstract
Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli/Proteus infections. Electron microscopy identified six phage types, with numerically T7-like phages dominating over T4-like phages. A metagenomic approach using taxonomical classification, reference mapping and de novo assembly identified 18 distinct phage types, including 7 genera of Podoviridae, 2 established and 2 proposed genera of Myoviridae, and 2 genera of Siphoviridae. De novo assembly yielded 7 contigs greater than 30. kb, including a 147-kb Myovirus genome and a 42-kb genome of a potentially new phage. Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure.
Keyword Bacteriophages
Escherichia coli
Fecal microbiota
Human phage therapy
Metagenomics
Safety
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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