Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis

Aranda-Sicilia, Maria Nieves, Trusov, Yuri, Maruta, Natsumi, Chakravorty, David, Zhang, Yuelin and Botella, Jose Ramon (2015) Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis. Journal of Plant Physiology, 188 44-48. doi:10.1016/j.jplph.2015.09.005

Author Aranda-Sicilia, Maria Nieves
Trusov, Yuri
Maruta, Natsumi
Chakravorty, David
Zhang, Yuelin
Botella, Jose Ramon
Title Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis
Journal name Journal of Plant Physiology   Check publisher's open access policy
ISSN 0176-1617
Publication date 2015-09-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.jplph.2015.09.005
Open Access Status Not Open Access
Volume 188
Start page 44
End page 48
Total pages 5
Place of publication Muenchen, Germany
Publisher Elsevier
Language eng
Subject 1314 Physiology
1102 Agronomy and Crop Science
1110 Plant Science
Abstract Heterotrimeric G proteins (G-proteins) are versatile signaling elements conserved in Eukaryotes. In animals G-proteins relay signals from 7-transmembrane spanning G protein-coupled receptors (GPCRs) to intracellular downstream effectors; however, the existence of GPCRs in plants is controversial. Contrastingly, a surplus of receptor-like kinases (RLKs) provides signal recognition at the plant cell surface. It is established that G proteins are involved in plant defense and suggested that they relay signals from defense-related RLKs. However, it is unclear how the signaling is conducted, as physical interaction between the RLKs and G proteins has not been demonstrated. Using yeast split-ubiquitin system and Bimolecular Fluorescence Complementation assays, we demonstrate physical interaction between the Gα, Gγ1 and Gγ2 subunits, and the defense-related RD-type receptor like kinases CERK1, BAK1 and BIR1. At the same time, no interaction was detected with the non-RD RLK FLS2. We hypothesize that G-proteins mediate signal transduction immediately downstream of the pathogenesis-related RLKs.
Keyword Heterotrimeric G proteins
Receptor-like kinases (RLKs)
Protein-protein interaction
Plant defence
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP1094152
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 20 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 13 Oct 2015, 10:34:45 EST by System User on behalf of Scholarly Communication and Digitisation Service