Experimental methods for studying drug uptake in the head and brain

Foster, K. A. and Roberts, M. S. (2000) Experimental methods for studying drug uptake in the head and brain. Current Drug Metabolism, 1 4: 333-356. doi:10.2174/1389200003338901


Author Foster, K. A.
Roberts, M. S.
Title Experimental methods for studying drug uptake in the head and brain
Journal name Current Drug Metabolism   Check publisher's open access policy
ISSN 1389-2002
Publication date 2000-01-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.2174/1389200003338901
Volume 1
Issue 4
Start page 333
End page 356
Total pages 24
Place of publication USA
Publisher Bentham Science
Language eng
Subject C3
320501 Pharmaceutical Sciences and Pharmacy
730118 Organs, diseases and abnormal conditions not elsewhere classified
Abstract A number of techniques have been developed to study the disposition of drugs in the head and, in particular, the role of the blood-brain barrier (BBB) in drug uptake. The techniques can be divided into three groups: in-vitro, in-vivo and in-situ. The most suitable method depends on the purpose(s) and requirements of the particular study being conducted. In-vitro techniques involve the isolation of cerebral endothelial cells so that direct investigations of these cells can be carried out. The most recent preparations are able to maintain structural and functional characteristics of the BBB by simultaneously culturing endothelial cells with astrocytic cells,The main advantages of the in-vitro methods are the elimination of anaesthetics and surgery. In-vivo methods consist of a diverse range of techniques and include the traditional Brain Uptake Index and indicator diffusion methods, as well as microdialysis and positron emission tomography. In-vivo methods maintain the cells and vasculature of an organ in their normal physiological states and anatomical position within the animal. However, the shortcomings include renal acid hepatic elimination of solutes as well as the inability to control blood flow. In-situ techniques, including the perfused head, are more technically demanding. However, these models have the ability to vary the composition and flow rate of the artificial perfusate. This review is intended as a guide for selecting the most appropriate method for studying drug uptake in the brain.
Keyword Biochemistry & Molecular Biology
Pharmacology & Pharmacy
Central-nervous-system
Alpha-aminoisobutyric-acid
Positron Emission Tomography
Cerebrospinal Fluid Barriers
Endothelial-cell-cultures
Perfused Rat-brain
In-situ Perfusion
Guinea-pig Brain
Blood-brain
Cytochrome-oxidase
Q-Index Code C3

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: School of Medicine Publications
 
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Created: Mon, 13 Aug 2007, 22:13:35 EST