Nitrate reduction by denitrifying anaerobic methane oxidizing microorganisms can reach a practically useful rate

Cai, Chen, Hu, Shihu, Guo, Jianhua, Shi, Ying, Xie, Guo-Jun and Yuan, Zhiguo (2015) Nitrate reduction by denitrifying anaerobic methane oxidizing microorganisms can reach a practically useful rate. Water Research, 87 211-217. doi:10.1016/j.watres.2015.09.026

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Author Cai, Chen
Hu, Shihu
Guo, Jianhua
Shi, Ying
Xie, Guo-Jun
Yuan, Zhiguo
Title Nitrate reduction by denitrifying anaerobic methane oxidizing microorganisms can reach a practically useful rate
Journal name Water Research   Check publisher's open access policy
ISSN 1879-2448
0043-1354
Publication date 2015-12-15
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.watres.2015.09.026
Open Access Status File (Author Post-print)
Volume 87
Start page 211
End page 217
Total pages 7
Place of publication London, United Kingdom
Publisher I W A Publishing
Language eng
Subject 2302 Ecological Modelling
2312 Water Science and Technology
2311 Waste Management and Disposal
2310 Pollution
Abstract Methane in biogas has been proposed to be an electron donor to facilitate complete nitrogen removal using denitrifying anaerobic methane oxidizing (DAMO) microorganisms in an anaerobic ammonium oxidation (anammox) reactor, by reducing the nitrate produced. However, the slow growth and the low activity of DAMO microorganisms cast a serious doubt about the practical usefulness of such a process. In this study, a previously established lab-scale membrane biofilm reactor (MBfR), with biofilms consisting of a coculture of DAMO and anammox microorganisms, was operated to answer if the DAMO reactor can achieve a nitrate reduction rate that can potentially be applied for wastewater treatment. Through progressively increasing nitrate and ammonium loading rates to the reactor, a nitrate removal rate of 684 +/- 10 mg-N L(-1)d(-1) was achieved after 453 days of operation. This rate is, to our knowledge, by far the highest reported for DAMO reactors, and far exceeds what is predicted to be required for nitrate removal in a sidestream (5.6-135 mg-N L(-1)d(-1)) or mainstream anammox reactor (3.2-124 mg-N L(-1)d(-1)). Mass balance analysis showed that the nitrite produced by nitrate reduction was jointly reduced by anammox bacteria at a rate of 354 3 mg-N L(-1)d(-1), accompanied by an ammonium removal rate of 268 2 mgN L(-1)d(-1), and DAMO bacteria at a rate of 330 +/- 9 mg-N L(-1)d(-1). This study shows that the nitrate reduction rate achieved by the DAMO process can be high enough for removing nitrate produced by anammox process, which would enable complete nitrogen removal from wastewater. (C) 2015 Elsevier Ltd. All rights reserved.
Formatted abstract
Methane in biogas has been proposed to be an electron donor to facilitate complete nitrogen removal using denitrifying anaerobic methane oxidizing (DAMO) microorganisms in an anaerobic ammonium oxidation (anammox) reactor, by reducing the nitrate produced. However, the slow growth and the low activity of DAMO microorganisms cast a serious doubt about the practical usefulness of such a process. In this study, a previously established lab-scale membrane biofilm reactor (MBfR), with biofilms consisting of a coculture of DAMO and anammox microorganisms, was operated to answer if the DAMO reactor can achieve a nitrate reduction rate that can potentially be applied for wastewater treatment. Through progressively increasing nitrate and ammonium loading rates to the reactor, a nitrate removal rate of 684 ± 10 mg-N L−1d−1 was achieved after 453 days of operation. This rate is, to our knowledge, by far the highest reported for DAMO reactors, and far exceeds what is predicted to be required for nitrate removal in a sidestream (5.6–135 mg-N L−1d−1) or mainstream anammox reactor (3.2–124 mg-N L−1d−1). Mass balance analysis showed that the nitrite produced by nitrate reduction was jointly reduced by anammox bacteria at a rate of 354 ± 3 mg-N L−1d−1, accompanied by an ammonium removal rate of 268 ± 2 mg-N L−1d−1, and DAMO bacteria at a rate of 330 ± 9 mg-N L−1d−1. This study shows that the nitrate reduction rate achieved by the DAMO process can be high enough for removing nitrate produced by anammox process, which would enable complete nitrogen removal from wastewater.
Keyword Anaerobic methane oxidation
Membrane biofilm reactor
Candidatus 'Methanoperedens nitroreducens'
Nitrate reduction rate
Nitrogen removal
Anammox
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DP120100163
DE130101401
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Advanced Water Management Centre Publications
 
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