Wound healing activity of standardized brazilin rich extract from Caesalpinia sappan heartwood

Nirmal, N. P., Prasad, R. G. S. V. and Keokitichai, S. (2014) Wound healing activity of standardized brazilin rich extract from Caesalpinia sappan heartwood. Journal of Chemical and Pharmaceutical Research, 6 10: 195-201.

Author Nirmal, N. P.
Prasad, R. G. S. V.
Keokitichai, S.
Title Wound healing activity of standardized brazilin rich extract from Caesalpinia sappan heartwood
Formatted title
Wound healing activity of standardized brazilin rich extract from Caesalpinia sappan heartwood
Journal name Journal of Chemical and Pharmaceutical Research   Check publisher's open access policy
ISSN 0975-7384
Publication date 2014-01-01
Sub-type Article (original research)
Open Access Status Not Open Access
Volume 6
Issue 10
Start page 195
End page 201
Total pages 7
Place of publication Rajasthan, India
Publisher Journal of Chemical and Pharmaceutical Research
Language eng
Abstract Wounds are common clinical entities of life which may be subacute or acute. Wound healing is a complex biochemical process where the cell structures are restored to normalcy, which depend on cell proliferation and migration, basically fibroblast cell.Plant polyphenols initiate wound healing process in natural way. The aim of this study was to evaluate the wound healing effect of standardized brazilin rich extract (BRE) from Casealpiniasappan heartwood. In vitro cell proliferation and migration of human fibroblast cell were determined by using scratch wound assay technique. Cytotoxicity, antioxidant and antibacterial activity of brazilin and BRE were also evaluated. Result indicated that brazilin and BRE had showed similar antioxidant and antibacterial activity (P > 0.05). Cytotoxicity study revealed that brazilin or BRE was nontoxic up to 500 µg/mL concentrations. Therefore, BRE can be used as potential bioactive replacer for brazilin owing to ease of production. Invitroscratch wound assay showed that BRE at 250 µg/ mL had highest cell proliferation and migration on day 2 of incubation. BRE treatment resulted in restoration of original cell number within 2 days when compared to control (P<0.05). Hence, BRE could be used as a potential herbal compound for the treatment of wound healing. BRE could be used as a replacer for brazilin owing to its similar biological activities and ease of low cost production.
Keyword Caesalpiniasappan heartwood
BRE
Biological activities
Human fibroblast cell
Wound healing
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes http://jocpr.com/vol6-iss10.html

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
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Created: Fri, 25 Sep 2015, 21:28:43 EST by Nilesh Nirmal on behalf of Centre for Nutrition and Food Sciences