Enhanced thermostability of a fungal alkaline protease by different additives

Nirmal, Nilesh P. and Laxman, R. Seeta (2014) Enhanced thermostability of a fungal alkaline protease by different additives. Enzyme Research, 2014 109303.1-109303.8. doi:10.1155/2014/109303


Author Nirmal, Nilesh P.
Laxman, R. Seeta
Title Enhanced thermostability of a fungal alkaline protease by different additives
Journal name Enzyme Research   Check publisher's open access policy
ISSN 2090-0414
2090-0406
Publication date 2014-01-01
Sub-type Article (original research)
DOI 10.1155/2014/109303
Open Access Status DOI
Volume 2014
Start page 109303.1
End page 109303.8
Total pages 8
Place of publication New York, NY, United States
Publisher Hindawi Publishing Corporation
Formatted abstract
A fungal strain (Conidiobolus brefeldianus MTCC 5184) isolated from plant detritus secreted a high activity alkaline protease. Thermostability studies of the fungal alkaline protease (FAP) revealed that the protease is stable up to 50°C with 40% residual activity after one hour. Effect of various additives such as sugars, sugar alcohols, polyols, and salts, on the thermostability of FAP was evaluated. Among the additives tested, glycerol, mannitol, xylitol, sorbitol, and trehalose were found to be very effective in increasing the stability of FAP, which was found to be concentration dependent. Fivefold increase in residual activity of FAP was observed in the presence of trehalose (50%) and sorbitol (50%) at 50°C for 4 h, compared to FAP without additive. Other additives like calcium at 20 mM and 10-15% ammonium sulphate showed lower stability improvement than trehalose and sorbitol. NaCl, MgCl2, K2HPO4, and glycine were found to be poor stabilizers and showed only a marginal improvement. PEG 6000 did not show any increase in stability but was found to be slightly inhibitory.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
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Created: Fri, 25 Sep 2015, 21:21:03 EST by Nilesh Nirmal on behalf of Examinations