Expression of inhibin α- and βA-subunit mRNA and protein in the fetal sheep ovary throughout gestation

Engelhardt H., Harkness L.M., Thomas G.B., Brooks A.N., McNeilly A.S. and Baird D.T. (1995) Expression of inhibin α- and βA-subunit mRNA and protein in the fetal sheep ovary throughout gestation. Molecular and Cellular Endocrinology, 107 2: 141-147. doi:10.1016/0303-7207(94)03435-V


Author Engelhardt H.
Harkness L.M.
Thomas G.B.
Brooks A.N.
McNeilly A.S.
Baird D.T.
Title Expression of inhibin α- and βA-subunit mRNA and protein in the fetal sheep ovary throughout gestation
Journal name Molecular and Cellular Endocrinology   Check publisher's open access policy
ISSN 0303-7207
Publication date 1995-02-01
Sub-type Article (original research)
DOI 10.1016/0303-7207(94)03435-V
Volume 107
Issue 2
Start page 141
End page 147
Total pages 7
Subject 1303 Specialist Studies in Education
1310 Endocrinology
1312 Molecular Biology
2712 Endocrinology, Diabetes and Metabolism
Abstract In adult sheep, inhibin expression in developing follicles appears to be associated with antrum formation. Our objective was to investigate using in situ hybridization and immunohistochemistry whether antral follicles present before birth in the sheep expressed mRNA or peptide for inhibin α- and βA-subunits. At days 70 and 100 when only primordial and primary follicles were present, there was no detectable mRNA or peptide for either inhibin subunit. By days 130 and 140 (term = 145 days), many secondary follicles were present, a proportion of which (∼50%) expressed detectable levels of α-subunit mRNA but not peptide. A number of antral follicles were present by this stage, all of which expressed α-subunit mRNA and peptide. Expression of βA-subunit mRNA and peptide was undetectable at all stages of gestation. Our results indicate that even in non-ovulatory follicles present before birth, expression of inhibin, at least the α-subunit, is developmentally linked with antrum formation.
Keyword Fetus
Follicle
Inhibin
mRNA
Ovary
Sheep
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Thu, 24 Sep 2015, 02:14:48 EST by Linda Harkness