Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel

Prokhorova, Tatyana A., Harkness, Linda M., Frandsen, Ulrik, Ditzel, Nicholas, Schroder, Henrik D., Burns, Jorge S. and Kassem, Moustapha (2009) Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. Stem Cells and Development, 18 1: 47-54. doi:10.1089/scd.2007.0266

Author Prokhorova, Tatyana A.
Harkness, Linda M.
Frandsen, Ulrik
Ditzel, Nicholas
Schroder, Henrik D.
Burns, Jorge S.
Kassem, Moustapha
Title Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel
Journal name Stem Cells and Development   Check publisher's open access policy
ISSN 1547-3287
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1089/scd.2007.0266
Open Access Status Not Open Access
Volume 18
Issue 1
Start page 47
End page 54
Total pages 8
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert, Inc. Publishers
Language eng
Subject 2720 Hematology
1309 Developmental Biology
1307 Cell Biology
Abstract When implanted into immunodeficient mice, human embryonic stem cells (hESCs) give rise to teratoma, tumor-like formations containing tissues belonging to all three germ layers. The ability to form teratoma is a sine qua non characteristic of pluripotent stem cells. However, limited data are available regarding the effects of implantation site and the methods employed for implantation on the success rate of teratoma formation. In this study, the rate of teratoma formation in immunodeficient mice was site dependent: subcutaneous (25-100%), intratesticular (60%), intramuscular (12.5%), and under the kidney capsule (100%). Co-injecting the hESCs with Matrigel increased subcutaneous teratoma formation efficiency from 25-40% to 80-100%. We did not observe site-specific differences in the teratoma composition at the histological level. However, subcutaneous teratomas were quite distinct, easy to remove, and caused minimal discomfort to the mice. Also, subcutaneous teratomas displayed larger proportion of solid tissues as opposed to cyst formation that dominated the teratomas formed at the other sites. Interestingly, a chromosomally abnormal hESCs with trisomy 20 formed teratomas where the ratio of differentiated to undifferentiated tissues was significantly decreased suggesting defective pluripotency of the cells. In conclusion, subcutaneous implantation of hESCs in presence of Matrigel appears to be the most efficient, reproducible, and the easiest approach for teratoma formation by hESCs. Also, teratoma formation can be employed to study the development defects exhibited by the chromosomally abnormal hESC lines.
Keyword Cell & Tissue Engineering
Medicine, Research & Experimental
Cell Biology
Research & Experimental Medicine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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