Procedures for derivation and characterisation of human embryonic stem cells from Odense, Denmark.

Harkness, Linda and Kassem, Moustapha (2012). Procedures for derivation and characterisation of human embryonic stem cells from Odense, Denmark.. In Human Embryonic Stem Cells Handbook (pp. 33-51) New York, NY United States: Humana Press. doi:10.1007/978-1-61779-794-1_3.


Author Harkness, Linda
Kassem, Moustapha
Title of chapter Procedures for derivation and characterisation of human embryonic stem cells from Odense, Denmark.
Title of book Human Embryonic Stem Cells Handbook
Place of Publication New York, NY United States
Publisher Humana Press
Publication Year 2012
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-61779-794-1_3.
Open Access Status
Series Methods in Molecular Biology, Methods and Protocols
ISBN 9781617797934
9781617797941
ISSN 1064-3745
Volume number 873
Chapter number 3
Start page 33
End page 51
Total pages 19
Total chapters 20
Language eng
Formatted Abstract/Summary
In 1998, a development occurred in stem cell biology with the first report of the derivation of a human embryonic stem cell (hESC) line. Since then a number of techniques have been used to derive and characterise hESCs. Here, we describe the derivation methods used by our laboratory for isolation of the ICM by immunosurgery and outgrowth of the whole blastocyst. We have added protocols for routine culture, passaging and cryopreservation of our hESC lines as well as the methods we have used for characterisation (flow cytometry, karyotyping, immunocytochemistry, in vitro and in vivo differentiation). Additionally, we have included gene sequences for PCR and an antibody list for immunocytochemistry.
Keyword Human embryonic stem cells
Derivation Characterisation
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Thu, 24 Sep 2015, 01:15:54 EST by Linda Harkness on behalf of Aust Institute for Bioengineering & Nanotechnology