A decade of metaproteomics: where we stand and what the future holds

Wilmes, Paul, Heintz-Buschart, Anna and Bond, Philip L. (2015) A decade of metaproteomics: where we stand and what the future holds. Proteomics, 15 20: 3409-3417. doi:10.1002/pmic.201500183

Author Wilmes, Paul
Heintz-Buschart, Anna
Bond, Philip L.
Title A decade of metaproteomics: where we stand and what the future holds
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9861
Publication date 2015-09-10
Year available 2015
Sub-type Article (original research)
DOI 10.1002/pmic.201500183
Open Access Status DOI
Volume 15
Issue 20
Start page 3409
End page 3417
Total pages 9
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Language eng
Abstract We are living through exciting times during which we are able to unravel the “microbial dark matter” in and around us through the application of high-resolution “meta-omics”. Metaproteomics offers the ability to resolve the major catalytic units of microbial populations and thereby allows the establishment of genotype-phenotype linkages from in situ samples. A decade has passed since the term “metaproteomics” was first coined and corresponding analyses were carried out on mixed microbial communities. Since then metaproteomics has yielded many important insights into microbial ecosystem function in the various environmental settings where it has been applied. Although initial progress in analytical capacities and resulting numbers of proteins identified was extremely fast, this trend slowed rapidly. Here, we discuss several representative metaproteomic investigations of activated sludge, acid mine drainage biofilms, freshwater and seawater microbial communities, soil, and human gut microbiota. By using these case studies, we highlight current challenges and possible solutions for metaproteomics to realize its full potential, i.e. to enable conclusive links between microbial community composition, physiology, function, interactions, ecology, and evolution in situ.
Keyword Integrated omics
Microbial community
Microbial systems ecology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 35 times in Thomson Reuters Web of Science Article | Citations
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