Effect of 5′UTR introns on gene expression in Arabidopsis thaliana

Chung, Betty Y. W., Simons, Cas, Firth, Andrew E., Brown, Chris M. and Hellens, Roger P. (2006) Effect of 5′UTR introns on gene expression in Arabidopsis thaliana. BMC Genomics, 7 . doi:10.1186/1471-2164-7-120

Author Chung, Betty Y. W.
Simons, Cas
Firth, Andrew E.
Brown, Chris M.
Hellens, Roger P.
Title Effect of 5′UTR introns on gene expression in Arabidopsis thaliana
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2006-05-19
Year available 2006
Sub-type Article (original research)
DOI 10.1186/1471-2164-7-120
Open Access Status DOI
Volume 7
Total pages 1
Place of publication LONDON
Language eng
Subject 2700 Medicine
1305 Biotechnology
1311 Genetics
Abstract Background: The majority of introns in gene transcripts are found within the coding sequences (CDSs). A small but significant fraction of introns are also found to reside within the untranslated regions (5′UTRs and 3′UTRs) of expressed sequences. Alignment of the whole genome and expressed sequence tags (ESTs) of the model plant Arabidopsis thaliana has identified introns residing in both coding and non-coding regions of the genome. Results: A bioinformatic analysis revealed some interesting observations: (1) the density of introns in 5′UTRs is similar to that in CDSs but much higher than that in 3′UTRs; (2) the 5′UTR introns are preferentially located close to the initiating ATG codon; (3) introns in the 5′UTRs are, on average, longer than introns in the CDSs and 3′UTRs; and (4) 5′UTR introns have a different nucleotide composition to that of CDs and 3′UTR introns. Furthermore, we show that the 5′UTR intron of the A. thaliana EFIα-A3 gene affects the gene expression and the size of the 5′UTR intron influences the level of gene expression. Conclusion: Introns within the 5′UTR show specific features that distinguish them from introns that reside within the coding sequence and the 3′UTR. In the EFIα-A3 gene, the presence of a long intron in the 5′UTR is sufficient to enhance gene expression in plants in a size dependent manner.
Keyword Biotechnology & Applied Microbiology
Genetics & Heredity
Biotechnology & Applied Microbiology
Genetics & Heredity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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