Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase

Abedinia, M, Layfield, R, Jones, SM, Nixon, PF and Mattick, JS (1992) Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochemical and Biophysical Research Communications, 183 3: 1159-1166. doi:10.1016/S0006-291X(05)80312-2


Author Abedinia, M
Layfield, R
Jones, SM
Nixon, PF
Mattick, JS
Title Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1992-03-31
Sub-type Article (original research)
DOI 10.1016/S0006-291X(05)80312-2
Volume 183
Issue 3
Start page 1159
End page 1166
Total pages 8
Language eng
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Transketolase is a key enzyme in the pentose-phosphate pathway which has been implicated in the latent human genetic disease, Wernicke-Korsakoff syndrome. Here we report the cloning and partial characterisation of the coding sequences encoding human transketolase from a human brain cDNA library. The library was screened with oligonucleotide probes based on the amino acid sequence of proteolytic fragments of the purified protein. Northern blots showed that the transketolase mRNA is approximately 2.2 kb, close to the minimum expected, of which approximately 60% was represented in the largest cDNA clone. Sequence analysis of the transketolase coding sequences reveals a number of homologies with related enzymes from other species.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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