Rapid concentration and sensitive detection of hookworm ova from wastewater matrices using a real-time PCR method

Gyawali, P., Sidhu, J. P. S., Ahmed, W., Jagals, P. and Toze, S. (2015) Rapid concentration and sensitive detection of hookworm ova from wastewater matrices using a real-time PCR method. Experimental Parasitology, 159 5-12. doi:10.1016/j.exppara.2015.08.009

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Author Gyawali, P.
Sidhu, J. P. S.
Ahmed, W.
Jagals, P.
Toze, S.
Title Rapid concentration and sensitive detection of hookworm ova from wastewater matrices using a real-time PCR method
Journal name Experimental Parasitology   Check publisher's open access policy
ISSN 1090-2449
0014-4894
Publication date 2015-12-01
Sub-type Article (original research)
DOI 10.1016/j.exppara.2015.08.009
Open Access Status File (Author Post-print)
Volume 159
Start page 5
End page 12
Total pages 8
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
The risk of human hookworm infections from land application of wastewater matrices could be high in regions with high hookworm prevalence. A rapid, sensitive and specific hookworm detection method from wastewater matrices is required in order to assess human health risks. Currently available methods used to identify hookworm ova to the species level are time consuming and lack accuracy. In this study, a real-time PCR method was developed for the rapid, sensitive and specific detection of canine hookworm (Ancylostoma caninum) ova from wastewater matrices. A. caninum was chosen because of its morphological similarity to the human hookworm (Ancylostoma duodenale and Necator americanus). The newly developed PCR method has high detection sensitivity with the ability to detect less than one A. caninum ova from 1 L of secondary treated wastewater at the mean threshold cycle (CT) values ranging from 30.1 to 34.3. The method is also able to detect four A. caninum ova from 1 L of raw wastewater and from ∼4 g of treated sludge with mean CT values ranging from 35.6 to 39.8 and 39.8 to 39.9, respectively. The better detection sensitivity obtained for secondary treated wastewater compared to raw wastewater and sludge samples could be attributed to sample turbidity. The proposed method appears to be rapid, sensitive and specific compared to traditional methods and has potential to aid in the public health risk assessment associated with land application of wastewater matrices. Furthermore, the method can be adapted to detect other helminth ova of interest from wastewater matrices.
Keyword Health risk assessment
Helminth
Hookworm
Real-time PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Public Health Publications
 
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