Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer

Li, Yingrui, Xu, Xun, Song, Luting, Hou, Yong, Li, Zesong, Tsang, Shirley, Li, Fuqiang, Im, Kate McGee, Wu, Kui, Wu, Hanjie, Ye, Xiaofei, Li, Guibo, Wang, Linlin, Zhang, Bo, Liang, Jie, Xie, Wei, Wu, Renhua, Jiang, Hui, Liu, Xiao, Yu, Chang, Zheng, Hancheng, Jian, Min, Nie, Liping, Wan, Lei, Shi, Min, Sun, Xiaojuan, Tang, Aifa, Guo, Guangwu, Gui, Yaoting, Cai, Zhiming, Li, Jingxiang, Wang, Wen, Lu, Zuhong, Zhang, Xiuqing, Bolund, Lars, Kristiansen, Karsten, Wang, Jian, Yang, Huanming, Dean, Michael and Wang, Jun (2012) Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer. GigaScience, 1 12: 1-14. doi:10.1186/2047-217X-1-12


Author Li, Yingrui
Xu, Xun
Song, Luting
Hou, Yong
Li, Zesong
Tsang, Shirley
Li, Fuqiang
Im, Kate McGee
Wu, Kui
Wu, Hanjie
Ye, Xiaofei
Li, Guibo
Wang, Linlin
Zhang, Bo
Liang, Jie
Xie, Wei
Wu, Renhua
Jiang, Hui
Liu, Xiao
Yu, Chang
Zheng, Hancheng
Jian, Min
Nie, Liping
Wan, Lei
Shi, Min
Sun, Xiaojuan
Tang, Aifa
Guo, Guangwu
Gui, Yaoting
Cai, Zhiming
Li, Jingxiang
Wang, Wen
Lu, Zuhong
Zhang, Xiuqing
Bolund, Lars
Kristiansen, Karsten
Wang, Jian
Yang, Huanming
Dean, Michael
Wang, Jun
Title Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer
Journal name GigaScience   Check publisher's open access policy
ISSN 2047-217X
Publication date 2012-08-14
Sub-type Article (original research)
DOI 10.1186/2047-217X-1-12
Open Access Status DOI
Volume 1
Issue 12
Start page 1
End page 14
Total pages 14
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background
Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, to date, the process of bladder cancer, which is one of the most common cancers in the world, remains unknown at a single-cell level.

Results
We carried out single-cell exome sequencing of 66 individual tumor cells from a muscle-invasive bladder transitional cell carcinoma (TCC). Analyses of the somatic mutant allele frequency spectrum and clonal structure revealed that the tumor cells were derived from a single ancestral cell, but that subsequent evolution occurred, leading to two distinct tumor cell subpopulations. By analyzing recurrently mutant genes in an additional cohort of 99 TCC tumors, we identified genes that might play roles in the maintenance of the ancestral clone and in the muscle-invasive capability of subclones of this bladder cancer, respectively.

Conclusions
This work provides a new approach of investigating the genetic details of bladder tumoral changes at the single-cell level and a new method for assessing bladder cancer evolution at a cell-population level.
Keyword Single-cell exome sequencing
Bladder cancer
Tumor evolution
Population genetics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 07 Sep 2015, 22:44:53 EST by Mr Mathew Carter on behalf of Institute for Molecular Bioscience