Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma

Sung, Wing-Kin, Zheng, Hancheng, Li, Shuyu, Chen, Ronghua, Liu, Xiao, Li, Yingrui, Lee, Nikki P., Lee, Wah H., Ariyaratne, Pramila N., Tennakoon, Chandana, Mulawadi, Fabianus H., Wong, Kwong F., Liu, Angela M., Poon, Ronnie T., Fan, Sheung Tat, Chan, Kwong L., Gong, Zhuolin, Hu, Yujie, Lin, Zhao, Wang, Guan, Zhang, Qinghui, Barber, Thomas D., Chou, Wen-Chi, Aggarwal, Amit, Hao, Ke, Zhou, Wei, Zhang, Chunsheng, Hardwick, James, Buser, Carolyn, Xu, Jiangchun, Kan, Zhengyan, Dai, Hongyue, Mao, Mao, Reinhard, Christoph, Wang, Jun and Luk, John M. (2012) Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma. Nature Genetics, 44 7: 765-769. doi:10.1038/ng.2295

Author Sung, Wing-Kin
Zheng, Hancheng
Li, Shuyu
Chen, Ronghua
Liu, Xiao
Li, Yingrui
Lee, Nikki P.
Lee, Wah H.
Ariyaratne, Pramila N.
Tennakoon, Chandana
Mulawadi, Fabianus H.
Wong, Kwong F.
Liu, Angela M.
Poon, Ronnie T.
Fan, Sheung Tat
Chan, Kwong L.
Gong, Zhuolin
Hu, Yujie
Lin, Zhao
Wang, Guan
Zhang, Qinghui
Barber, Thomas D.
Chou, Wen-Chi
Aggarwal, Amit
Hao, Ke
Zhou, Wei
Zhang, Chunsheng
Hardwick, James
Buser, Carolyn
Xu, Jiangchun
Kan, Zhengyan
Dai, Hongyue
Mao, Mao
Reinhard, Christoph
Wang, Jun
Luk, John M.
Title Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma
Journal name Nature Genetics   Check publisher's open access policy
ISSN 1061-4036
Publication date 2012-01-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1038/ng.2295
Open Access Status Not Open Access
Volume 44
Issue 7
Start page 765
End page 769
Total pages 5
Place of publication New York, NY United States
Publisher Nature Publishing Group
Language eng
Abstract To survey hepatitis B virus (HBV) integration in liver cancer genomes, we conducted massively parallel sequencing of 81 HBV-positive and 7 HBV-negative hepatocellular carcinomas (HCCs) and adjacent normal tissues. We found that HBV integration is observed more frequently in the tumors (86.4%) than in adjacent liver tissues (30.7%). Copy-number variations (CNVs) were significantly increased at HBV breakpoint locations where chromosomal instability was likely induced. Approximately 40% of HBV breakpoints within the HBV genome were located within a 1,800-bp region where the viral enhancer, X gene and core gene are located. We also identified recurrent HBV integration events (in ≥4 HCCs) that were validated by RNA sequencing (RNA-seq) and Sanger sequencing at the known and putative cancer-related TERT, MLL4 and CCNE1 genes, which showed upregulated gene expression in tumor versus normal tissue. We also report evidence that suggests that the number of HBV integrations is associated with patient survival.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Institute for Molecular Bioscience - Publications
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