Characterisation of Australian isolates of Fusarium oxysporum f. sp cubense by DNA fingerprinting analysis

Gerlach, K. S., Bentley, S., Moore, N. Y., Pegg, K. G. and Aitken, E. A. B. (2000) Characterisation of Australian isolates of Fusarium oxysporum f. sp cubense by DNA fingerprinting analysis. Australian Journal of Agricultural Research, 51 8: 945-953. doi:10.1071/AR99172

Author Gerlach, K. S.
Bentley, S.
Moore, N. Y.
Pegg, K. G.
Aitken, E. A. B.
Title Characterisation of Australian isolates of Fusarium oxysporum f. sp cubense by DNA fingerprinting analysis
Journal name Australian Journal of Agricultural Research   Check publisher's open access policy
ISSN 0004-9409
Publication date 2000-01-01
Year available 2000
Sub-type Article (original research)
DOI 10.1071/AR99172
Open Access Status Not yet assessed
Volume 51
Issue 8
Start page 945
End page 953
Total pages 9
Place of publication Australia
Publisher CSIRO Publishing
Language eng
Subject C1
270402 Plant Physiology
620205 Tropical fruit
Abstract Genetic variation among Australian isolates of the fungus Fusarium oxysporum f. sp. cubense (Foc), which causes Fusarium wilt in banana, was examined using DNA amplification fingerprinting (DAF). Ninety-four isolates which represented Races 1, 2, 3, and 4, and vegetative compatibility groups (VCGs) 0120, 0124, 0125, 0128, 0129, 01211, 01213/16, and 01220 were analysed. The genetic relatedness among isolates within each VCG, and between the 8 different VCGs of Foc present in Australia was determined. The DNA fingerprint patterns were VCG-specific, with each VCG representing a unique genotype. The genetic similarity among isolates within each VCG ranged from 97% to 100%. Among the different VCGs of Foc, 3 major clusters were distinguished which corresponded with race. All Race 1 and 2 isolates (VCGs 0124, 0125, 0128, and 01220) were closely related and clustered together, the Race 3 isolates from Heliconia clustered separately, and all Race 4 isolates (VCGs 0120, 0129, 01211, and 01213/16) clustered together. Fifteen isolates from Alstonville, NSW, were characterised because although they were classified as Race 2 based on their recovery from cooking banana cultivars, they belonged in VCG 0124, which had previously contained only Race 1 isolates. The occurrence of more than one race within a VCG means that vegetative compatibility grouping cannot be used to assign pathotype to pathogenic race as previously thought. It was possible to distinguish the Race 1 and Race 2 isolates within VCG 0124 using DNA fingerprinting, as each race produced a unique DNA fingerprint pattern. Among the Australian isolates, DNA fingerprinting analysis identified 9 different VCGs and genotypes of Foc.
Keyword Agriculture, Multidisciplinary
Polymerase Chain Reaction
Vegetative Compatibility Group
Vegetative Compatibility Groups
Cavendish Bananas
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
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Created: Mon, 13 Aug 2007, 22:01:51 EST