Induction of heme-oxygenase-1 (HO-1) does not enhance adiponectin production in human adipocytes: Evidence against a direct HO-1 - Adiponectin axis

Yang, Mengliu, Kimura, Masaki, Ng, Choaping, He, Jingjing, Keshvari, Sahar, Rose, Felicity J, Barclay, Johanna L and Whitehead, Jonathan P (2015) Induction of heme-oxygenase-1 (HO-1) does not enhance adiponectin production in human adipocytes: Evidence against a direct HO-1 - Adiponectin axis. Molecular and Cellular Endocrinology, 413 209-216. doi:10.1016/j.mce.2015.06.034


Author Yang, Mengliu
Kimura, Masaki
Ng, Choaping
He, Jingjing
Keshvari, Sahar
Rose, Felicity J
Barclay, Johanna L
Whitehead, Jonathan P
Title Induction of heme-oxygenase-1 (HO-1) does not enhance adiponectin production in human adipocytes: Evidence against a direct HO-1 - Adiponectin axis
Journal name Molecular and Cellular Endocrinology   Check publisher's open access policy
ISSN 0303-7207
1872-8057
Publication date 2015-09-15
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.mce.2015.06.034
Open Access Status Not Open Access
Volume 413
Start page 209
End page 216
Total pages 8
Place of publication Shannon, Clare Ireland
Publisher Elsevier Ireland
Language eng
Formatted abstract
Adiponectin is a salutary adipokine and hypoadiponectinemia is implicated in the aetiology of obesity-related inflammation and cardiometabolic disease making therapeutic strategies to increase adiponectin attractive. Emerging evidence, predominantly from preclinical studies, suggests induction of heme-oxygenase-1 (HO-1) increases adiponectin production and reduces inflammatory tone. Here, we aimed to test whether induction of HO-1 enhanced adiponectin production from mature adipocytes. Treatment of human adipocytes with cobalt protoporphyrin (CoPP) or hemin for 24–48 h increased HO-1 expression and activity without affecting adiponectin expression and secretion. Treatment of adipocytes with TNFα reduced adiponectin secretion and increased expression and secretion of additional pro-inflammatory cytokines, IL-6 and MCP-1, as well as expression of sXBP-1, a marker of ER stress. HO-1 induction failed to reverse these effects. These results demonstrate that induction of HO-1 does not directly enhance adiponectin production or ameliorate the pro-inflammatory effects of TNFα and argue against a direct HO-1 – adiponectin axis.
Keyword Adiponectin
HO-1
Inflammation
Therapeutic
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
Official 2016 Collection
School of Medicine Publications
 
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Created: Tue, 25 Aug 2015, 23:18:43 EST by Johanna Barclay on behalf of Mater Research Institute-UQ