Binding of starch fragments to the starch branching enzyme: implications for developing slower-digesting starch

Go, Rob Marc, Mark, Alan E., Malde, Alpeshkumar K. and Gilbert, Robert G. (2015) Binding of starch fragments to the starch branching enzyme: implications for developing slower-digesting starch. Biomacromolecules, 16 8: 2475-2481. doi:10.1021/acs.biomac.5b00710


Author Go, Rob Marc
Mark, Alan E.
Malde, Alpeshkumar K.
Gilbert, Robert G.
Title Binding of starch fragments to the starch branching enzyme: implications for developing slower-digesting starch
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1526-4602
1525-7797
Publication date 2015-08-10
Sub-type Article (original research)
DOI 10.1021/acs.biomac.5b00710
Open Access Status DOI
Volume 16
Issue 8
Start page 2475
End page 2481
Total pages 7
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract Molecular weight distributions of starch branches affect functional properties, which can be controlled by engineering starch branching enzymes (SBEs). Molecular dynamics and docking approaches are used to examine interactions between SBE and starch fragments. In the native protein, three residues formed stable interactions with starch fragments in the central binding region; these residues may play key roles in substrate recognition. Fragments containing 5–12 glucose units interacted more tightly with SBE than smaller fragments, suggesting a minimal functional fragment size of 5, agreeing with experiment. Effects of three different point mutations on interactions with maltopentaose in the central binding region correlated well with experiment. Simulations indicate that SBE may template formation of the crystalline lamellae characteristic of native starch, consistent with the observation that crystalline lamellae formed by starch in a plant, are not necessarily the state of lowest free energy. The methodology will help develop starches with optimized functional properties.
Keyword Starch branching enzymes (SBEs)
Molecular weight distribution (MWD)
Molecular dynamic simulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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