Contrasting association of circulating sCD14 with insulin sensitivity in non-obese and morbidly obese subjects

de Courten, Barbora, Moreno-Navarrete, Jose Maria, Lyons, Jasmine, Soldatos, Georgia, de Courten, Maximilian, Dougherty, Sonia, Forbes, Josephine and Fernandez-Real, Jose Manuel (2015) Contrasting association of circulating sCD14 with insulin sensitivity in non-obese and morbidly obese subjects. Molecular Nutrition and Food Research, 60 1: 103-109. doi:10.1002/mnfr.201500102

Author de Courten, Barbora
Moreno-Navarrete, Jose Maria
Lyons, Jasmine
Soldatos, Georgia
de Courten, Maximilian
Dougherty, Sonia
Forbes, Josephine
Fernandez-Real, Jose Manuel
Title Contrasting association of circulating sCD14 with insulin sensitivity in non-obese and morbidly obese subjects
Journal name Molecular Nutrition and Food Research   Check publisher's open access policy
ISSN 1613-4125
Publication date 2015-01-01
Year available 2016
Sub-type Article (original research)
DOI 10.1002/mnfr.201500102
Open Access Status Not Open Access
Volume 60
Issue 1
Start page 103
End page 109
Total pages 7
Place of publication Weinheim, Germany
Publisher Wiley-VCH Verlag
Language eng
Formatted abstract
In experimental studies, moderate to high concentrations of sCD14 (serum cluster of differentiation 14 protein) prevent lipopolysaccharide (LPS)-induced systemic inflammation, while low concentrations may promote inflammation. Given that metabolic endotoxemia is thought to initiate high-fat diet-induced insulin resistance, we explored the association between sCD14 concentrations and insulin sensitivity in humans.

Methods and results
Healthy non-obese (n = 12, BMI 26 ± 5y), obese (n = 11, BMI 33.45 ± 3.2) and morbidly obese participants (n = 38, BMI 45 ± 7) underwent measurement of body composition (dual energy X-ray absorptiometry) and a hyperinsulinemic-euglycemic clamp to measure insulin sensitivity (M value). Circulating sCD14 concentrations were measured by ELISA. Non-obese participants had lower circulating sCD14 concentrations compared to obese (p = 0.03). Circulating sCD14 concentrations were positively associated with percent body fat, waist circumference and white blood cell count and negatively associated with insulin sensitivity. In contrast, circulating sCD14 were positively associated with insulin sensitivity in morbidly obese participants. In regression analysis, insulin sensitivity (r = 0.52, p = 0.004) and fasting triglycerides (r = 0.49, p = 0.005) contributed independently to circulating sCD14 variance after controlling for age, sex and BMI in these morbidly obese subjects.

These findings suggest that circulating sCD14 concentrations, through its compensatory (in non-obese subjects) or buffering role (in morbidly obese subjects), could exert an important role in modulating insulin sensitivity.
Keyword Adiposity
Insulin resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
Official 2016 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
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