Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa

Sanchez-Calabuig, M. J., de la Fuente, J., Laguna-Barraza, R., Beltran-Brena, P., Martinez-Nevado, E., Johnston, S. D., Rizos, D., Gutierrez-Adan, A. and Perez-Gutierrez, J. F. (2015) Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa. Theriogenology, 84 6: 983-994. doi:10.1016/j.theriogenology.2015.06.001

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Author Sanchez-Calabuig, M. J.
de la Fuente, J.
Laguna-Barraza, R.
Beltran-Brena, P.
Martinez-Nevado, E.
Johnston, S. D.
Rizos, D.
Gutierrez-Adan, A.
Perez-Gutierrez, J. F.
Title Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa
Formatted title
Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
1879-3231
Publication date 2015-01-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2015.06.001
Open Access Status File (Author Post-print)
Volume 84
Issue 6
Start page 983
End page 994
Total pages 12
Place of publication Philadelphia, PA United States
Publisher Elsevier
Language eng
Formatted abstract
Assisted reproductive technologies are of great importance for increasing the genetic diversity in captive animals. The use of bovine or murine oocytes in heterologous IVF provides advantages compared to homologous IVF in nondomestic animals, such as the accessibility to oocytes and the availability of well-developed in vitro maturation systems. The aim of this study was to determine the heterologous IVF parameters using cryopreserved dolphin spermatozoa and zona-intact bovine or murine oocytes and to examine the nuclear chromatin status of the dolphin spermatozoa. All the processes involved in the fertilization including embryo cleavage were observed by confocal microscopy and hybrid embryo formation was confirmed by polymerase chain reaction. Heterologous bovine IVF showed no polyspermy, lower percentages of pronuclear formation, and a lower cleavage rate compared to homologous IVF group (34.8% vs. 89.3%). Heterologous murine IVF showed a lower cleavage rate than homologous IVF (9.6% vs. 77.1%). With respect to dolphin sperm chromatin, it was more stable, i.e. more resistant to EDTA-SDS decondensation than the bovine sperm chromatin. This study revealed the stability of the dolphin sperm chromatin and the ability of the dolphin spermatozoa to penetrate zona-intact bovine and murine oocytes, leading to hybrid embryo formation.
Keyword Heterologous IVF
Bottlenose dolphin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
 
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