Sequential increase in Egr-1 and AP-1 DNA binding activity in the dentate gyrus following the induction of long-term potentiation

Williams, JM, Beckmann, AM, Mason-Parker, SE, Abraham, WC, Wilce, PA and Tate, WP (2000) Sequential increase in Egr-1 and AP-1 DNA binding activity in the dentate gyrus following the induction of long-term potentiation. Molecular Brain Research, 77 2: 258-266. doi:10.1016/S0169-328X(00)00061-9


Author Williams, JM
Beckmann, AM
Mason-Parker, SE
Abraham, WC
Wilce, PA
Tate, WP
Title Sequential increase in Egr-1 and AP-1 DNA binding activity in the dentate gyrus following the induction of long-term potentiation
Journal name Molecular Brain Research   Check publisher's open access policy
ISSN 0169-328X
Publication date 2000-01-01
Sub-type Article (original research)
DOI 10.1016/S0169-328X(00)00061-9
Open Access Status Not yet assessed
Volume 77
Issue 2
Start page 258
End page 266
Total pages 9
Language eng
Subject 1312 Molecular Biology
2804 Cellular and Molecular Neuroscience
Abstract Establishment of long-term potentiation (LTP) at perforant path synapses is highly correlated with increased expression of Egr and AP-1 transcription factors in rat dentate gyrus granule cells. We have investigated whether increased transcription factor levels are reflected in increased transcription factor activity by assessing Egr and AP-I DNA binding activity using gel shift assays. LTP produced an increase in binding to the Egr element, which was NMDA receptor-dependent and correlated closely with our previously reported increase in Egr-1 (zif/268) protein levels. Supershift analysis confirmed involvement of Egr-1, but not Egr-2 in the DNA binding activity. AP-1 DNA binding was also rapidly elevated in parallel with protein levels, however, the peak increase in activity was delayed until 4 h, a time point when we have previously shown that only jun-D protein was elevated. These data indicate that binding of Egr-1 and AP-1 to their response elements is increased in two phases. This may result in activation of distinct banks of target genes which contribute to the establishment of persistent LTP. (C) 2000 Elsevier Science B.V. All rights reserved.
Keyword Neurosciences
Transcription Factor
Gel Shift Assay
Long-term Potentiation
Immediate-early Gene
Finger Transcription Factor
C-jun
Hippocampal-neurons
Glutamate-receptor
Nervous-system
Messenger-rna
Expression
Fos
Promoter
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Mon, 13 Aug 2007, 21:47:44 EST