Health attributes of an endemic orchid from Eastern Anatolia, Dactylorhiza chuhensis Renz&Taub. - in vitro investigations

Dalar, Abdullah, Guo, Yu, Esim, Nevzat, Bengu, Aydin Sukru and Konczak, Izabela (2015) Health attributes of an endemic orchid from Eastern Anatolia, Dactylorhiza chuhensis Renz&Taub. - in vitro investigations. Journal of Herbal Medicine, 5 2: 77-85. doi:10.1016/j.hermed.2015.02.001

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Author Dalar, Abdullah
Guo, Yu
Esim, Nevzat
Bengu, Aydin Sukru
Konczak, Izabela
Title Health attributes of an endemic orchid from Eastern Anatolia, Dactylorhiza chuhensis Renz&Taub. - in vitro investigations
Formatted title
Health attributes of an endemic orchid from Eastern Anatolia, Dactylorhiza chuhensis Renz&Taub. - in vitro investigations
Journal name Journal of Herbal Medicine   Check publisher's open access policy
ISSN 2210-8041
2210-8033
Publication date 2015-06-01
Sub-type Article (original research)
DOI 10.1016/j.hermed.2015.02.001
Open Access Status File (Author Post-print)
Volume 5
Issue 2
Start page 77
End page 85
Total pages 9
Place of publication Munich, Germany
Publisher Elsevier
Language eng
Formatted abstract
Phytochemical composition and potential health attributes of Dactylorhiza chuhensis Renz&Taub., an endemic orchid from Eastern Anatolia, were investigated. Lyophilized methanol-based extracts obtained from leaf, flower, stem and tuber were investigated for the presence of phenolic compounds [Folin–Ciocalteu assay and high performance liquid chromatography analysis (HPLC)], antioxidant capacities [ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assay] and enzyme-inhibitory activities [lipase, α-amylase, α-glucosidase and angiotensin converting enzyme (ACE)]. The tuber, used as a traditional remedy and utilized by the pharmaceutical industry, had the lowest content of total phenolics, inferior antioxidant and enzyme-inhibitory activities. The highest phenolic content (44.9 ± 0.8 mg GAE/g DW) was exhibited by the leaf extract, which also showed superior reducing (736.8 ± 16.2 μmol Fe2+/g DW) and oxygen radical scavenging capacities (2715.8 ± 83.5 μmol Trolox E/g DW). The inhibitory activities of the leaf extract toward α-amylase, α-glucosidase and ACE were moderate. Applied at non-toxic concentrations, the leaf extract effectively reduced accumulation of nitric oxide (NO) in lipopolysaccharide (LPS)-activated hepatocellular carcinoma (HepG2) cells. Further studies towards potential utilization of D. chuhensis leaf as a source of physiologically active phytochemcials are justified.
Keyword Antioxidant
Dactylorhiza chuhensis Renz&Taub.
Enzyme-inhibitory activity
Total phenolics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2016 Collection
 
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