Complete genome sequence and intracellular protein localization of Datura yellow vein nucleorhabdovirus

Dietzgen, Ralf G., Innes, David J. and Bejerman, Nicolas (2015) Complete genome sequence and intracellular protein localization of Datura yellow vein nucleorhabdovirus. Virus Research, 205 7-11. doi:10.1016/j.virusres.2015.05.001


Author Dietzgen, Ralf G.
Innes, David J.
Bejerman, Nicolas
Title Complete genome sequence and intracellular protein localization of Datura yellow vein nucleorhabdovirus
Journal name Virus Research   Check publisher's open access policy
ISSN 1872-7492
0168-1702
Publication date 2015-07-02
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.virusres.2015.05.001
Open Access Status Not yet assessed
Volume 205
Start page 7
End page 11
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract A limited number of plant rhabdovirus genomes have been fully sequenced, making taxonomic classification, evolutionary analysis and molecular characterization of this virus group difficult. We have for the first time determined the complete genome sequence of 13,188 nucleotides of Datura yellow vein nucleorhabdovirus (DYVV). DYVV genome organization resembles that of its closest relative, Sonchus yellow net virus (SYNV), with six ORFs in antigenomic orientation, separated by highly conserved intergenic regions and flanked by complementary 3′ leader and 5′ trailer sequences. As is typical for nucleorhabdoviruses, all viral proteins, except the glycoprotein, which is targeted to the endoplasmic reticulum, are localized to the nucleus. Nucleocapsid (N) protein, matrix (M) protein and polymerase, as components of nuclear viroplasms during replication, have predicted strong canonical nuclear localization signals, and N and M proteins exclusively localize to the nucleus when transiently expressed as GFP fusions. As in all nucleorhabdoviruses studied so far, N and phosphoprotein P interact when co-expressed, significantly increasing P nuclear localization in the presence of N protein. This research adds to the list of complete genomes of plant-infecting rhabdoviruses, provides molecular tools for further characterization and supports classification of DYVV as a nucleorhabdovirus closely related to but with some distinct differences from SYNV.
Keyword Genome structure
Nucleorhabdovirus
Plant virus
Protein localization
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2016 Collection
 
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