Intracellular microenvironment-responsive label-free autofluorescent nanogels for traceable gene delivery

Shi, Bingyang, Zhang, Hu, Qiao, Shi Zhang, Bi, Jingxiu and Dai, Sheng (2014) Intracellular microenvironment-responsive label-free autofluorescent nanogels for traceable gene delivery. Advanced Healthcare Materials, 3 11: 1839-1848. doi:10.1002/adhm.201400187

Author Shi, Bingyang
Zhang, Hu
Qiao, Shi Zhang
Bi, Jingxiu
Dai, Sheng
Title Intracellular microenvironment-responsive label-free autofluorescent nanogels for traceable gene delivery
Journal name Advanced Healthcare Materials   Check publisher's open access policy
ISSN 2192-2640
Publication date 2014-11-01
Year available 2014
Sub-type Article (original research)
DOI 10.1002/adhm.201400187
Open Access Status Not Open Access
Volume 3
Issue 11
Start page 1839
End page 1848
Total pages 10
Place of publication Weinheim, Germany
Publisher Wiley-VCH Verlag
Language eng
Formatted abstract
Gene therapy presents a unique opportunity for the treatment of genetic diseases, but the lack of multifunctional delivery systems has hindered its clinical applications. Here, a new delivery vector, autofluorescent polyethyleneimine (PEI) nanogels, for highly efficient and traceable gene delivery is developed. Different from commercial high-molecular-weight PEI, the cationic nanogels are noncytotoxic and able to be fragmented due to their unique intracellular microenvironment-responsive structures. The biodegradable nanogels can effectively load plasmid DNA (pDNA), and the self-assembled polyplexes can be cleaved after cellular uptake to improve gene transfection efficiency. Most importantly, the nanogels and the nanogel/pDNA polyplexes are autofluorescent. The fluorescence is stable in blood plasma and responsive to the intracellular microenvironment. The breakup of the nanogels or polyplexes leads to the loss of fluorescence, and thus the gene delivery and carrier biodegradation processes can be monitored. The reported multifunctional system demonstrates excellent biocompatibility, high transfection efficiency, responsive biodegradability, controlled gene release, label-free and simultaneous fluorescence tracking, which will provide a new platform for future scientific investigation and practical implications in gene therapy.
Keyword Label-free autofluorescence
Intracellular environment
Gene delivery carriers
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DP110102877
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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