Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates

Fan, Ben, Carvalhais, Lilia C., Becker, Anke, Fedoseyenko, Dmitri, von Wiren, Nicolaus and Borriss, Rainer (2012) Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates. BMC Microbiology, 12 116.1-116.13. doi:10.1186/1471-2180-12-116


Author Fan, Ben
Carvalhais, Lilia C.
Becker, Anke
Fedoseyenko, Dmitri
von Wiren, Nicolaus
Borriss, Rainer
Title Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates
Formatted title
Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates
Journal name BMC Microbiology   Check publisher's open access policy
ISSN 1471-2180
Publication date 2012-06-21
Sub-type Article (original research)
DOI 10.1186/1471-2180-12-116
Open Access Status DOI
Volume 12
Start page 116.1
End page 116.13
Total pages 13
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates.

Results: Bacillus amyloliquefaciens FZB42 is a well-studied Gram-positive PGPR. To obtain a comprehensive overview of FZB42 gene expression in response to maize root exudates, microarray experiments were performed. A total of 302 genes representing 8.2% of the FZB42 transcriptome showed significantly altered expression levels in the presence of root exudates. The majority of the genes (261) was up-regulated after incubation of FZB42 with root exudates, whereas only 41 genes were down-regulated. Several groups of the genes which were strongly induced by the root exudates are involved in metabolic pathways relating to nutrient utilization, bacterial chemotaxis and motility, and non-ribosomal synthesis of antimicrobial peptides and polyketides.

Conclusions: Here we present a transcriptome analysis of the root-colonizing bacterium Bacillus amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Thu, 18 Jun 2015, 05:15:22 EST by Lilia Costa Carvalhais on behalf of School of Agriculture and Food Sciences