Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition

Beatson, Scott A., Ben Zakour, Nouri L., Totsika, Makrina, Forde, Brian M., Watts, Rebecca E., Mabbett, Amanda N., Szubert, Jan M., Sarkar, Sohinee, Phan, Minh-Duy, Peters, Kate M., Petty, Nicola K., Alikhan, Nabil-Fareed, Sullivan, Mitchell J., Gawthorne, Jayde A., Stanton-Cook, Mitchell, Nguyen Thi Khanh Nhu, Chong, Teik Min, Yin, Wai-Fong, Chan, Kok-Gan, Hancock, Viktoria, Ussery, David W., Ulett, Glen C. and Schembri, Mark A. (2015) Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition. Infection and Immunity, 83 5: 1749-1764. doi:10.1128/IAI.02810-14

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ362700_OA.pdf Full text (open access) application/pdf 3.71MB 0

Author Beatson, Scott A.
Ben Zakour, Nouri L.
Totsika, Makrina
Forde, Brian M.
Watts, Rebecca E.
Mabbett, Amanda N.
Szubert, Jan M.
Sarkar, Sohinee
Phan, Minh-Duy
Peters, Kate M.
Petty, Nicola K.
Alikhan, Nabil-Fareed
Sullivan, Mitchell J.
Gawthorne, Jayde A.
Stanton-Cook, Mitchell
Nguyen Thi Khanh Nhu
Chong, Teik Min
Yin, Wai-Fong
Chan, Kok-Gan
Hancock, Viktoria
Ussery, David W.
Ulett, Glen C.
Schembri, Mark A.
Title Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition
Formatted title
Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2015-05-01
Year available 2015
Sub-type Article (original research)
DOI 10.1128/IAI.02810-14
Open Access Status File (Publisher version)
Volume 83
Issue 5
Start page 1749
End page 1764
Total pages 16
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli responsible for >80% of all cases. One extreme of UTI is asymptomatic bacteriuria (ABU), which occurs as an asymptomatic carrier state that resembles commensalism. To understand the evolution and molecular mechanisms that underpin ABU, the genome of the ABU E. coli strain VR50 was sequenced. Analysis of the complete genome indicated that it most resembles E. coli K-12, with the addition of a 94-kb genomic island (GI-VR50-pheV), eight prophages, and multiple plasmids. GI-VR50-pheV has a mosaic structure and contains genes encoding a number of UTI-associated virulence factors, namely, Afa (afimbrial adhesin), two autotransporter proteins (Ag43 and Sat), and aerobactin. We demonstrated that the presence of this island in VR50 confers its ability to colonize the murine bladder, as a VR50 mutant with GI-VR50-pheV deleted was attenuated in a mouse model of UTI in vivo.We established that Afa is the island-encoded factor responsible for this phenotype using two independent deletion (Afa operon and AfaE adhesin) mutants. E. coli VR50afa and VR50afaE displayed significantly decreased ability to adhere to human bladder epithelial cells. In the mouse model of UTI, VR50afa and VR50afaE displayed reduced bladder colonization compared to wild-type VR50, similar to the colonization level of the GI-VR50-pheV mutant. Our study suggests that E. coli VR50 is a commensal-like strain that has acquired fitness factors that facilitate colonization of the human bladder.
Keyword Immunology
Infectious Diseases
Immunology
Infectious Diseases
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 511224
FT100100662
APP1090456
DE130101169
H-50001-A000027
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 10 Jun 2015, 19:53:54 EST by Anthony Yeates on behalf of School of Chemistry & Molecular Biosciences