Localization of N-acetyltransferases NAT1 and NAT2 in human tissues

Windmill, Kelly F., Gaedigk, Andrea, Hall, Pauline de la M., Samaratunga, Hema, Grant, Denis M. and McManus, Michael E. (2000) Localization of N-acetyltransferases NAT1 and NAT2 in human tissues. Toxicological Sciences, 54 1: 19-29. doi:10.1093/toxsci/54.1.19

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Author Windmill, Kelly F.
Gaedigk, Andrea
Hall, Pauline de la M.
Samaratunga, Hema
Grant, Denis M.
McManus, Michael E.
Title Localization of N-acetyltransferases NAT1 and NAT2 in human tissues
Journal name Toxicological Sciences   Check publisher's open access policy
ISSN 1096-0929
1096-6080
Publication date 2000-03-01
Year available 2000
Sub-type Article (original research)
DOI 10.1093/toxsci/54.1.19
Open Access Status Not Open Access
Volume 54
Issue 1
Start page 19
End page 29
Total pages 11
Place of publication San Diego, CA, United States
Publisher Oxford University Press
Language eng
Subject 270200 Genetics
270100 Biochemistry and Cell Biology
C1
060107 Enzymes
Abstract Human acetyl coenzyme A-dependent N-acetyltransferase (EC 2.3.1.5) (NAT) catalyzes the biotransformation of a number of arylamine and hydrazine compounds. NAT isozymes are encoded at 2 loci; one encodes NAT1, formerly known as the monomorphic form of the enzyme, while the other encodes the polymorphic NAT2, which is responsible for individual differences in the ability to acetylate certain compounds. Human epidemiological studies have suggested an association between the acetylator phenotype and particular cancers such as those of the bladder and colon. In the present study, NAT1- and NAT2-specific riboprobes were used in hybridization histochemistry studies to localize NAT1 and NAT2 mRNA sequences in formalin-fixed, paraffin-embedded human tissue sections. Expression of both NAT1 and NAT2 mRNA was observed in liver, gastrointestinal tract tissues (esophagus, stomach, small intestine, and colon), ureter, bladder, and lung. In extrahepatic tissues, NAT1 and NAT2 mRNA expression was localized to intestinal epithelial cells, urothelial cells, and the epithelial cells of the respiratory bronchioles. The observed heterogeneity of NAT1 and NAT2 mRNA expression between human tissue types may be of significance in assessing their contribution to known organ-specific toxicities of various arylamine drugs and carcinogens.
Keyword Toxicology
N-acetyltransferase
Carcinogenesis
Hybridization Histochemistry
Liver
Bladder
Human Gastrointestinal Tissues
Glutathione S-transferases
Human Uroepithelial Cells
Human Urinary-bladder
Immunohistochemical Localization
Acetylator Genotype
Monoclonal-antibody
Gene-expression
Lung-cancer
Human Liver
Q-Index Code C1
Institutional Status UQ
Additional Notes This is a pre-copying editing, author produced PDF of an article accepted for publication in Toxicological Sciences following peer review. The definitive publisher-authenticated version Kelly F. Windmill, Andrea Gaedigk, Pauline de la M. Hall, Hema Samaratunga, Denis M. Grant, and Michael E. McManus (2000) Localization of N-Acetyltransferases NAT1 and NAT2 in Human Tissues. Toxicological Sciences, 54: 19-29 is available online at : http://toxsci.oxfordjournals.org Copyright 2000 Oxford Journals. All rights Reserved.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Science Publications
 
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Created: Mon, 13 Aug 2007, 21:38:41 EST