Activated complement factor 3 is associated with liver fat and liver enzymes: the CODAM study

Wlazlo, Nick, van Greevenbroek, Marleen M. J., Ferreira, Isabel, Jansen, Eugene H., Feskens, Edith J., van der Kallen, Carla J., Schalkwijk, Casper G., Bravenboer, Bert and Stehouwer, Coen D. (2013) Activated complement factor 3 is associated with liver fat and liver enzymes: the CODAM study. European Journal of Clinical Investigation, 43 7: 679-688. doi:10.1111/eci.12093

Author Wlazlo, Nick
van Greevenbroek, Marleen M. J.
Ferreira, Isabel
Jansen, Eugene H.
Feskens, Edith J.
van der Kallen, Carla J.
Schalkwijk, Casper G.
Bravenboer, Bert
Stehouwer, Coen D.
Title Activated complement factor 3 is associated with liver fat and liver enzymes: the CODAM study
Journal name European Journal of Clinical Investigation   Check publisher's open access policy
ISSN 0014-2972
Publication date 2013-07-01
Year available 2013
Sub-type Article (original research)
DOI 10.1111/eci.12093
Volume 43
Issue 7
Start page 679
End page 688
Total pages 10
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Background: The complement system may be involved in the pathogenesis of alcoholic and nonalcoholic liver disease, although studies in humans are scarce. For this reason, we investigated whether circulating levels of activated complement factor 3 (C3a) were associated with hepatic steatosis and hepatocellular damage.

Materials and methods: Plasma C3a, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT) were determined in 523 individuals (61% men, age 59 ± 7 years). Liver enzymes (LEs) were standardized and compiled into a LE score. Liver fat content was estimated using a predictive equation that has recently been validated with magnetic resonance spectrometry. Cross-sectional associations between C3a and liver fat or LE s were investigated with multiple linear regression analyses, stratified in no-to-moderate vs. heavy alcohol consumers (men: > 30 g/day; women: > 20 g/day).

Results: C3a was associated with liver fat percentage both in the no-to-moderate (β = 0·223; 95%CI 0·036; 0·409) and in the heavy alcohol consumers (β = 0·632; 95%CI 0·259-1·004; P-interaction = 0·047). C3a was also associated with the LE score in heavy alcohol consumers (β = 0·917; 95%CI 0·443-1·392), but not in no-to-moderate alcohol consumers (β = 0·042; 95%CI -0·198 to 0·281; P-interaction = 0·001).

Conclusions: C3a levels, as a marker of complement activation, were associated with liver fat content and hepatocellular injury, at least in subjects who consume considerable amounts of alcohol daily.
Keyword Alcoholic fatty liver disease
Complement system
Nonalcoholic fatty liver disease
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 29 May 2015, 09:48:04 EST by Isabel Ferreira on behalf of School of Public Health